Optimization of 3D hyaluronic acid-alginate hydrogel-based culture system for chondrocytes from osteoarthritis patient

Name: Nuchanart Suntornnont

Organization : Chulalongkorn University. Faculty of Science

Name: Supansa Yodmuang

Organization : Chulalongkorn University. Faculty of Medicine

Name: Rachaneekorn Tammachote

Organization : Chulalongkorn University. Faculty of Science

Email : ratchaneekorn.t@chula.ac.th

keyword: Pellet culture

LCSH: Cartilage cells

; Alginate hydrogel

; 3D cell culture systems

; Chondrocyte

Abstract: Three-dimensional (3D) cell culture systems have been applied to several research designed to mimic the microenvironment of tissues, leading cells in these platforms to represent tissue more accurately than those grown in two-dimensional (2D) culture systems. Moreover, osteoarthritis (OA) is associated with excessive cartilage degradation, but the majority of pharmacological clinical studies were significantly effective under traditional 2D culture systems. Thus, these highlights represent the urgent requirement to enhance the understanding of the pathophysiology, therapeutic targets, and practical model development for OA drug selection. Therefore, this study aims to optimize the 3D cell culture conditions under non-scaffold-based and scaffold-based models on OA chondrocyte viability. The optimal model was evaluated using live/dead and morphological assay. The results of the non-scaffold-based culture system as a pellet culture model was optimized to the optimal condition of pellet size and rotation time under centrifugation using live/dead assay to 50,000 cell/pellet under centrifugation at 300x g for 5 min. In relation to the scaffold-based culture system as the hyaluronic acid -alginate (HA-Alg) hydrogel-based model, the hydrogel construction under the HA-Alg crosslinking on the construct size over the formulating obstructions was optimized to 250 μl. Moreover, cells under the HA-Alg hydrogel-based model were illustrated with the highest accuracy on cell viability and tissue mimetic morphology based on live/dead and morphological assay. In conclusion, the HA-Alg hydrogel-based 3D culturing conditions were optimized as a possible strategy for preserving the viability of OA chondrocytes and tendency development as an appropriate platform for the future application of OA drug screening

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2023

Modified: 2567-05-01

Issued: 2024-04-25

บทความ/Article

application/pdf

BibliograpyCitation : In Rangsit University. The 8th RSU International Research Conference on Sciences and Technology 2023 (RSUCON 2023) (pp.9-17). Pathum Thani : Rangsit University

eng

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