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Peerapong Pornwongthong. Immobilization of cellulase on zinc oxide deposited on zeolite pellets for enzymatic saccharification of cellulose. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2023.
Immobilization of cellulase on zinc oxide deposited on zeolite pellets for enzymatic saccharification of cellulose
Name: EOM, Tokla
Organization :
King Mongkut's University of Technology North Bangkok. Faculty of Applied Science
Name: Jantiya Isanapong
Organization :
King Mongkut's University of Technology North Bangkok. Faculty of Applied Science
Name: Pisist Kumnorkaew
Organization :
National Science and Technology Development Agency. National Nanotechnology Center
Name: Krisanavej Songthanasak
Organization :
King Mongkut's University of Technology North Bangkok. Faculty of Applied Science
Name: Peerapong Pornwongthong
Organization :
King Mongkut's University of Technology North Bangkok. Faculty of Applied Science
keyword:
Enzymatic hydrolysis
LCSH:
Biocatalysis
LCSH:
Enzyme kinetics
Abstract:
The consumption of fossil fuels to fulfill the global energy demand can cause global warming
issues. Renewable energy, i.e., bioethanol, from lignocellulosic biomass, is a promising source of
alternative energy to fossil fuels. The conversion of lignocellulosic biomass into bioethanol requires the
release of fermentable sugars during the saccharification process using cellulase. However, the utilization
of this enzyme on an industrial scale is not feasible due to its difficult separation, instability, and high cost.
Here, we present a method for cellulase immobilization on functionalized zinc oxide prepared from either
zinc nitrate hexahydrate (ZnO(I)) or zinc acetate dihydrate (ZnO(II)) solutions on zeolite (ZEO) pellets.
The immobilized cellulase on ZnO-ZEO structures was characterized by scanning electron microscopy, Xray
diffraction spectroscopy, and Fourier transform infrared spectroscopy. The immobilization efficiencies
of immobilized cellulase either on ZnO(I)-ZEO or ZnO(II)-ZEO were determined as 58.17 ± 0.75% and
55.51 ± 0.81%, respectively. The immobilized cellulase on ZnO-ZEO was capable of catalyzing
microcrystalline cellulose breakdown, releasing reducing sugars. The immobilized cellulase on these
structures could be recycled up to four repetitive runs. Based on kinetic data, both the Michaelis constants
(Km) and maximum reaction velocity (Vmax) of the immobilized cellulase on the ZnO-ZEO structures were
lower than those of free cellulase. This suggests that immobilized cellulase has a higher affinity toward the
substrate, but a lower reaction rate than the free enzyme
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2023
Modified:
2024-01-11
Issued:
2024-01-11
บทความ/Article
application/pdf
BibliograpyCitation :
In King Mongkut's University of Technology North Bangkok. Science and Technology Research Institute. 2023 Research, Innovation, and Innovation Congress (RI2C 2023) (Iss32). Bangkok : Science and Technology Research Institute
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.036177 วินาที
EOM, Tokla
| Title | Contributor | Type |
|---|---|---|
| Immobilization of cellulase on zinc oxide deposited on zeolite pellets for enzymatic saccharification of cellulose
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ EOM, Tokla;Jantiya Isanapong ;Pisist Kumnorkaew;Krisanavej Songthanasak ;Peerapong Pornwongthong | บทความ/Article |
Jantiya Isanapong
| Title | Contributor | Type |
|---|---|---|
| Immobilization of cellulase on zinc oxide deposited on zeolite pellets for enzymatic saccharification of cellulose
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ EOM, Tokla;Jantiya Isanapong ;Pisist Kumnorkaew;Krisanavej Songthanasak ;Peerapong Pornwongthong | บทความ/Article |
Pisist Kumnorkaew
| Title | Contributor | Type |
|---|---|---|
| Life cycle assessment of nano-silica coating for solar photovoltaic panel
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Pakon Laopreecha;Viganda Varabuntoonvit;Pisist Kumnorkaew | บทความ/Article | |
| Immobilization of cellulase on zinc oxide deposited on zeolite pellets for enzymatic saccharification of cellulose
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Tokla EOM;Jantiya Isanapong;Pisist Kumnorkaew;Krisanavej Songthanasak;Peerapong Pornwongthong | บทความ/Article | |
| Immobilization of cellulase on zinc oxide deposited on zeolite pellets for enzymatic saccharification of cellulose
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ EOM, Tokla;Jantiya Isanapong ;Pisist Kumnorkaew;Krisanavej Songthanasak ;Peerapong Pornwongthong | บทความ/Article |
Krisanavej Songthanasak
| Title | Contributor | Type |
|---|---|---|
| Immobilization of cellulase on zinc oxide deposited on zeolite pellets for enzymatic saccharification of cellulose
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ EOM, Tokla;Jantiya Isanapong ;Pisist Kumnorkaew;Krisanavej Songthanasak ;Peerapong Pornwongthong | บทความ/Article |
Peerapong Pornwongthong
| Title | Contributor | Type |
|---|---|---|
| Nutritional properties of nine microgreens consumed in Thailand
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Jareerat Chunthawodtiporn.;Thanyatorn Kuengsaard.;Benya Manochai.;Peerapong Pornwongthong. | บทความ/Article | |
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| Immobilization of cellulase on zinc oxide deposited on zeolite pellets for enzymatic saccharification of cellulose
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Tokla EOM;Jantiya Isanapong;Pisist Kumnorkaew;Krisanavej Songthanasak;Peerapong Pornwongthong | บทความ/Article | |
| Conversion of lignocellulose residue obtained from biorefinery stream to electricity by microbial fuel cell
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Keerthi Katam;Maythee Saisriyoot;Supacharee Roddecha;Peerapong Pornwongthong;Malinee Sriariyanun | บทความ/Article | |
| Immobilization of cellulase on zinc oxide deposited on zeolite pellets for enzymatic saccharification of cellulose
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ EOM, Tokla;Jantiya Isanapong ;Pisist Kumnorkaew;Krisanavej Songthanasak ;Peerapong Pornwongthong | บทความ/Article |