Abstract:
Rosy Danio (Danio roesus) are freshwater fish with high nutritional values but low in essential fatty acids when compared to marine fish. However, these essential fatty acids are found in many oil plants especially perilla. Therefore, this study aimed to examine the effects of perilla oil supplements on growth and the accumulation of omega-3 fatty acids in the Rosy Danio. The completely randomized design (CRD) trial was divided into 4 treatments with 3 replications, consisting of food with different concentrations of perilla oil at 0 (control), 4, 8, and 12%. Before the experiment started, the average weight of fish was at 0.22±0.05 g. 100 fish/pond were cultured in a cement pond (diameter 1 m) for 90 days. At the end of the experiment, Rosy Danio in the group fed a diet with perilla oil at 4% had significantly higher length gain, weight gain, specific growth rate, and feed conversation ratio (p < 0.05). The survival rate, Rosy Danio in the group fed a diet with perilla oil at 4% was the highest. They were significantly different from those feds with perilla oil at 8% and 12% (p < 0.05), but not significantly from the control (p > 0.05). B/C ratio of Rosy Danio fed a diet with perilla oil 4% had the highest value, but not significantly (p > 0.05) from all treatments. Omega-3 accumulated in the fish at the end the trend increased with the amount of perilla oil mixed in food; the values were 242.56±7.87, 461.59±9.84, 792.49±10.73 and 1,117.84±7.60 mg/g respectively. Therefore, our results suggest that dietary supplementation with 4% perilla oil would be suitable for Rosy Danio feeding. However, the knowledge gained can be applied to other fish species in the future