แจ้งเอกสารไม่ครบถ้วน, ไม่ตรงกับชื่อเรื่อง หรือมีข้อผิดพลาดเกี่ยวกับเอกสาร ติดต่อที่นี่ ==>
หากไม่มีอีเมลผู้รับให้กรอก thailis-noc@uni.net.th
หากไม่มีอีเมลผู้รับให้กรอก thailis-noc@uni.net.th
Sasikarn Kongsompong. Inhibitory effect of abalone embryo digested fractions (AEDFs) on the expression of murine fibroblast collagenases (MMP-1, MMP-8, MMP-13). Master's Degree(Biology). Kasetsart University. Office of the University Library. : Kasetsart University, 2017.
Inhibitory effect of abalone embryo digested fractions (AEDFs) on the expression of murine fibroblast collagenases (MMP-1, MMP-8, MMP-13)
Name: Sasikarn Kongsompong
Classification :.LCCS: QL430.5.H34
LCSH:
Abalones
LCSH:
Abalone culture
LCSH:
Fibroblasts
LCSH:
Collagenases
Abstract:
The Haliotis diversicolor has long been known as a valuable food source in Asian countriesincluding Thailand. In aquaculture industries only healthy embryos will be selected, the unwanted are quite excessive that could be used as components in skin care products or supplements which claim to help anti-skin aging. There are several reports on the ability of abalone to prevent the collagen degradation by collagenases (MMP1, MMP-8, and MMP-13), however the effect remains unclear. Therefore, this experiment aims to study distribution of abalone embryo hydrolysate in different molecular mass ranges using Viva-spin® columns and found that more than 60% of the digested fraction was smaller than 50 kDa. These small peptides are potential source of bioactive peptides. Effect of the abalone embryo digested fractions (<3, 3-10, 10-30 and >30 kDa) and abalone embryo undigested crude on the growth and viability of murine fibroblast cell line was examined using the MTT assay. The result of cell viability showed no different which indicated that all fragments were not toxic to the fibroblast cells. Effect of the abalone embryo digested fractions (< 5, 5-50, 50-100 and >100 kDa) and abalone embryo digested crude on murine fibroblast collagenase activity at mRNA and protein levels was conducted using RT-qPCR and zymography techniques. Result found that the fraction 2 down-regulated MMP-8 expression and the fraction 3 decreased MMP-13 expression at mRNA level. While crude decreased the band D collagenase activity at protein level. As the results, the abalone embryos are safe and can reduce the expression of collagenase which can be potential source for dietary supplements and skin care products to prevent collagen degradation.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Name: Pramote Chumnanpuen
Role:
Thesis Advisor
Name: Teerasak E-kobon
Role:
Thesis Co-Advisor
Created:
2017
Modified:
2023-06-29
Issued:
2023-06-29
วิทยานิพนธ์/Thesis
application/pdf
URL: https://kasetsart.idm.oclc.org/login?url=https://www.lib.ku.ac.th/KUthesis/2560/sasikarn-kon-all.pdf
CallNumber:
QL430.5.H34.S27
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Biology
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.033717 วินาที
Sasikarn Kongsompong
| Title | Contributor | Type |
|---|---|---|
| Inhibitory effect of abalone embryo digested fractions (AEDFs) on the expression of murine fibroblast collagenases (MMP-1, MMP-8, MMP-13)
มหาวิทยาลัยเกษตรศาสตร์ Sasikarn Kongsompong | Pramote Chumnanpuen Teerasak E-kobon | วิทยานิพนธ์/Thesis |
| Anti-melanogenic effect of abalone biomimetic peptides on murine melanoma cell line
มหาวิทยาลัยเกษตรศาสตร์ Sasikarn Kongsompong | Pramote Chumnanpuen | วิทยานิพนธ์/Thesis |
Pramote Chumnanpuen
Teerasak E-kobon