Prevalence and characterization of extended-spectrum beta-lactamase producing Escherichia coli and Kiebsiella pneumoniae isolated from clinical samples in a Tertiary Care Center

Name: Phanvasri Saengsuwan

Organization : Prince of Songkla University. Faculty of Medicine

Email : sphanvas@medicine.psu.ac.th

Name: Chonticha Romyasanit

Organization : Walailak Universiy. School of Allied Health Sciences

Name: Phoomjai Somns

Organization : Prince of Songkla University. Faculty of Medicine

keyword: Klebsiella pneumoniae infections

LCSH: Escherichia coli infections

LCSH: Beta lactamases

MeSH: Drug Resistance, Microbial

LCSH: Clinical microbiology

Abstract: The goal of the study was to determine the prevalence and genetic characteristics of extended- spectrum beta-lactamase (ESBL)-producing bacteria isolated from clinical samples in a tertiary care hospital in southern Thailand. ESBL isolates (n = 102) were collected from clinical specimens from January 2021 to August 2021. The double-disc synergistic test was used to assess ESBL production among these clinical isolates. ESBL encoding genes were examined by multiplex PCR. Of the 102 ESBL (23 E. coli and 79 K. pneumoniae), 46 8% and 53.9% were from male and female patients, respectively. The majority were isolated from urine (44.1%), followed by body fluid (26.5%), blood (18.6%), and tissue (10.8%). ESBL - producing K. pneumoniae was the most frequently diagnosed in the medical ward (36.3%), followed by the surgical ward (33.3%), and the operating room (11.8%), respectively. The antibiotic susceptibility test showed that 100% of the isolates were resistant to ampicillin, cefotaxime, ceftazidime, ceftriaxone, and cefuroxime. Ertapenem and meropenem were active against 99% of ESBL-producers. It was also discovered that the rate of multidrug resistance for ESBL was 58.8% and 45.1% for extremely drug resistant ESBL producers. The most common genes among ESBL producers were blaTeM (62.7%), blasHv (49.0%), and blacrx-M (7.8%), while blaGEs, blaveB, and blapER were not found in any of them. Interestingly, co-harboring genes of blatem and blasHv were found in all isolates of ESBL- producing K. pneumoniae. To our knowledge, this is the first study to demonstrate a high prevalence of multidrug resistance and the presence of more than one ESBL variant gene in a tertiary care hospital in southern Thailand, highlighting the challenge of treatment.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2023

Modified: 2023-06-23

Issued: 2023-06-23

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BibliograpyCitation : In Mae Fah Luang University. School of Science. The Pure and Applied Chemistry International Conference 2023 (PACCON 2023) (pp.664-669). Chiang Rai : Mae Fah Luang University

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