Physiochemical characterization, biocompatibility and controlled release properties of poly (caprolactone)-bioactive glass-based composite scaffolds for bone tissue engineering

Name: Khamsone Keothongkham

LCSH: Kasetsart University -- Dissertations. Ph.D. (Physics) 2017

Classification :.LCCS: QC173.4.C63

LCSH: Kasetsart University. Department of Physics -- Dissertations

LCSH: Composite materials

LCSH: Bioactive glasses

LCSH: Biomedical materials

LCSH: Polycaprolactone

LCSH: polyvinyl alcohol

LCSH: Bone regeneration

LCSH: Tissue engineering

LCSH: Tissue scaffolds

Abstract: Composite materials having the mechanical and biological properties similar to those of human bones are need for bone regeneration and repair. This study composites were made by incorporating bioactive glass (BG) into poly(caprolactone) (PCL) based composite scaffolds. For bioactive glass incorporated poly(caprolactone)-poly(vinyl alcohol) (PVA) (PCLPVA) matrix. Composites with different BG contents of 10, 25 and 50 wt% were fabricated by an in-situ blending method. Physicochemical properties measurement found that composite with 50 wt% BG in the PCLPVA matrix exhibited the best mechanical properties (compressive strength and young’s modulus up to 32.26±1.42 MPa and 530.91 MPa, respectively). We investigate the effect of the BG content in cell adhesion, proliferation and osteogenic activity of UMR-106 cells grown on the scaffolds using in vitro cell culture. The composite scaffold having 25% BG showed a significant increase in their cell adhesion capability and faster cell proliferation. They also exhibited cell adhesion and spreading morphology after 5 days of culturing. For these reasons we chose to attach the bone morphogenetic proteins (BMP-2) to this composite. The results showed the significant improvement in the UMR-106 cells adhesion, enhancement in osteogenic differentiation and osteoinductivity of this composite. In addition, we have also fabricated the chitosan (Chi)-poly(caprolactone) (PCL)/BG composite scaffolds in the ratios of Chi:PCL/BG (1:3, 1:1 and 3:1; wt/wt). The results indicated that the highest compressive strength of 39.86±0.50 MPa was obtained for 1:1 Chi:PCL/BG scaffold. While the lowest compressive strength is found to be in the case of 3:1 Chi:PCL/BG because of poor mechanical properties of chitosan. Mineralization study demonstrated apatite particles formation on the composite scaffolds surface after incubated in simulated body fluid (SBF) for 7 days. In vitro cell availability and proliferation tests confirmed the osteoblast attachment and growth on the Chi:PCLx/BG surface after cultured for 3, 5 and 7 days. To test it bioactivity, 1:1 Chi:PCL/BG of polymers weight ratio was chosen for MTT and ALP assay on UMR-106 cells. The results indicated that the UMR-106 cells were viable and had higher ALP activity. The study result on the BSA protein controlled release, revealed that the release rate quite low.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Weeraphat Pon-on

Role: Thesis Advisor

Created: 2017

Modified: 2023-06-16

Issued: 2023-06-16

วิทยานิพนธ์/Thesis

application/pdf

URL: https://kasetsart.idm.oclc.org/login?url=https://www.lib.ku.ac.th/KUthesis/2560/khamsone-keo-all.pdf

CallNumber: QC173.4.C63.K43

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Physics

Grantor: Kasetsart University

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