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Wipa Chantarawong. Effects of cadmium on human retinal pigment epithelial cells and melanocytes. Doctoral Degraee(Bioscience). Kasetsart University. Office of the University Library. : Kasetsart University, 2016.
Effects of cadmium on human retinal pigment epithelial cells and melanocytes
Name: Wipa Chantarawong
Classification :.LCCS: QP671.V5
LCSH:
Rhodopsin
LCSH:
Melanocytes
LCSH:
Epithelial cells
LCSH:
Cadmium
Abstract:
Cadmium (Cd) is one of the environmental toxicants that have been implicated in the pathogenesis of a range of chronic disorders, including age-related macular degeneration (AMD) and hearing loss. The functions of retina and cochlea are maintained by RPE cells and melanocytes, respectively and proliferation of both cells is regulated by microphthalmia-associated transcription factor (MITF). The purpose of the present study is to determine the cytotoxicity effects of cadmium on cell viability and MITF expression in the RPE cell lines (ARPE-19 and D407), which mainly expressed MITF-H, and human melanocytes (normal human epidermal melanocytes (NHEM) and melanoma cells (HMV-II)), which mainly express MITF-M. Cadmium exposure caused cell growth-rate reduction and cell growth arrest in ARPE-19 and D407 cells evaluated by trypan blue exclusion and MTT assay. The results from quantitative real time RT-PCR and western blot analysis showed that cadmium significantly decreased levels of MITF-M protein and its mRNA in NHEM and HMV-II cells. Moreover, cadmium also decreased tyrosinase mRNA levels, the key enzyme of melanogenesis that is regulated by MITF-M in NHEM and HMV-II cells. However, cadmium did not affect on the levels of total MITF protein, constituting mainly MITF-H in RPE cells. These results suggest that MITF-M may be a molecular target of cadmium toxicity in melanocyte.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Name: Kantimanee Pradermwong
Role:
Thesis Advisor
Created:
2016
Modified:
2023-06-14
Issued:
2023-06-14
วิทยานิพนธ์/Thesis
application/pdf
URL: https://kasetsart.idm.oclc.org/login?url=https://www.lib.ku.ac.th/KUthesis/2559/wipa-cha-all.pdf
CallNumber:
QP671.V5.W57
eng
DegreeName: Doctor of Philosophy
Descipline: Bioscience
Grantor: Kasetsart University
Level: Doctoral Degraee
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.032617 วินาที
Wipa Chantarawong
| Title | Contributor | Type |
|---|---|---|
| Effects of cadmium on human retinal pigment epithelial cells and melanocytes
มหาวิทยาลัยเกษตรศาสตร์ Wipa Chantarawong | Kantimanee Pradermwong | วิทยานิพนธ์/Thesis |
Kantimanee Pradermwong
| Title | Creator | Type and Date Create |
|---|---|---|
| Effects of cadmium on human retinal pigment epithelial cells and melanocytes
มหาวิทยาลัยเกษตรศาสตร์ Kantimanee Pradermwong | Wipa Chantarawong | วิทยานิพนธ์/Thesis |
| Immunostimulating and anti-inflammatory activities of four indigenous Thai russula mushrooms on RAW 264.7 cells
มหาวิทยาลัยเกษตรศาสตร์ Kantimanee Pradermwong;Prapaipat Klungsupya | Weerasak Taengphan | วิทยานิพนธ์/Thesis |
| Biological effects of Vitex glabrata RBr crude extract on TK6, L929 and MCF-7 cell lines
มหาวิทยาลัยเกษตรศาสตร์ Kantimanee Pradermwong | Saraphorn Intarasam | วิทยานิพนธ์/Thesis |
| Evaluation of chronic toxicity of vernonia cinerea Less extract on wistar rats
มหาวิทยาลัยเกษตรศาสตร์ Kantimanee Pradermwong | Thularath Toommakorn | วิทยานิพนธ์/Thesis |