Development of elite rice lines with broad spectrum of blast disease resistance by gene pyramiding using SSR markers

Name: Dipti Jayantrao Wankhade

LCSH: Kasetsart University -- Dissertations. Ph.D. (Tropical Agriculture) 2016

Classification :.LCCS: SB608.R5

LCSH: Kasetsart University. Faculty of Agriculture -- Dissertations

LCSH: Rice -- Breeding

LCSH: Rice -- Disease and pest resistance -- genetic aspects

LCSH: Rice blast disease

Abstract: Rice blast caused by fungus Pyricularia oryzae is one of the major factors reducing rice production in Thailand. The Jao Hom Nin (JHN) and IR64 rice varieties have broad spectrum resistance to blast pathogens. Qignizhan (CH1) variety has high yield potential, but it is susceptible to blast disease in Thailand. This study focused on improving the resistance towards Pyricularia oryzae in rice lines by three-way crossing of JHN, IR64 and CH1. This three-way crossing was implemented to introgress highly blast resistant QTLs into a single genotype. Marker-assisted selection (MAS) was used to identify four quantitative trait loci (QTLs) in preliminary pyramided lines (pre-PYL) from F3 lines. To select the desired plants which had resistance towards eight Thai isolates of Pyricularia oryzae from North, North East and Central of Thailand, genotypic screening was done with five Simple Sequence Repeat (SSR) markers involving RM212, RM319, RM144, RM208, and RM179. This resulted in seven pre-PYL from F3 lines carrying QTLs on chr.1, chr.11, chr.2, and chr.12 identified by MAS that exhibited both genotypic and phenotypic broad spectrum resistance of blast disease. Yield test was done to evaluate the agronomic traits and yield components of the 20 F5 lines. The result of grain yield per plant at 14% moisture content, the line 1-1-4-1 was shown high yield potential (5.13g/plant). Total grain weight and 12 lines showed non-significant difference to check varieties (PTT1, RD31 and CNT1). The results of broad spectrum resistance (BSR) and virulence index (V.I.) of 20 F6 lines with 7 check varieties showed resistance towards the blast pathogens and KDML105 variety showed highly susceptible to all 20 isolates. Thus, gene pyramiding lines can be used directly and be valuable source of resistance in future plant breeding programs.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Chalermpol Phumichai

Role: Thesis Advisor

Name: Tanee Sreewongchai

Role: Thesis Co-Advisor

Name: Pasajee Kongsil

Role: Thesis Co-Advisor

Created: 2016

Modified: 2023-06-14

Issued: 2023-06-14

วิทยานิพนธ์/Thesis

application/pdf

URL: https://kasetsart.idm.oclc.org/login?url=https://www.lib.ku.ac.th/KUthesis/2559/dipti-jay-all.pdf

CallNumber: SB608.R5.W36

eng

DegreeName: Doctor of Philosophy

Descipline: Tropical Agriculture

Grantor: Kasetsart University

Level: Doctoral Degraee

©copyrights Kasetsart University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	dipti-jay-all.pdf	5.22 MB

ใช้เวลา

0.027744 วินาที