Effect of culture age and cryoprotectants on the viability of freeze-dried Lactobacillus paracasei AN3 and its antagonistic activity against Aspergillus flavus

Name: Passanun Phanthuma-opas

Organization : Chulalongkorn University. Faculty of Science

Name: Supat Chareonpornwattana

Organization : Chulalongkorn University. Faculty of Science

Name: Cheewanun Dachoupakan Sirisomboon

Organization : Chulalongkorn University. Faculty of Science

Email : cheewanun.d@chula.ac.th

keyword: Cryoprotectants

ThaSH: Aspergillus flavus

ThaSH: Freeze-drying

ThaSH: Lactobacillus paracasei

Abstract: The objectives of this study were to determine the effect of culture age and different cryoprotectants on the viability of Lactobacillus paracasei AN3 after the freeze-drying process and to evaluate its biocontrol efficiency against growth and aflatoxin B1 production of Aspergillus flavus. The highest percentage of survival cells (120.04 + 2.73 percent) was obtained after freeze-drying, when skim milk (10 percent,w/v) was used as a cryoprotectant. It provided high cell viability until three months of storage at ambient temperature. The culture age did not have a significant effect on survival cells. For evaluation of antagonistic activities, all freeze-dried cells of L. paracasei AN3 could maintain the ability to inhibit the growth and reduce the AFB1 production of A. flavus.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2021

Modified: 2023-06-07

Issued: 2023-06-07

บทความ/Article

application/pdf

BibliograpyCitation : In Thai Society for Biotechnology. Thai Society for Biotechnology and International Conference Online (TSB Online 2021) (pp.486-495). Bangkok : Thai Society for Biotechnology

eng

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