Lab-made spectrometer for determination of tetracycline in pharmaceuticals

Name: N. Bawornnithichaiyakul

Organization : Thammasat University. Faculty of Science and Technology

Name: Hemper, A.

Organization : Thammasat University. Faculty of Science and Technology

Name: N. Youngvises

Organization : Thammasat University. Faculty of Science and Technology

Email : nyoungvises@hotmail.com, napaporn@tu.ac.th

LCSH: Drugs -- Analysis

LCSH: Tetracycline

LCSH: Diagnosis, Laboratory -- Equipment and supplies

LCSH: Mass spectrometers

Abstract: The spectrophotometer is a common instrument in the chemical laboratory and is used in various applications such as in industry, agriculture, education, and in pharmaceuticals. However, most of them were not portable for field analysis and also expensive and complicated for invention in the general laboratory. In this work, the simple spectrometer was invented in our laboratory with simple components such as a laser pointer, light-dependent resistor (LDR), plastic holder, and multi-meter. The blue-light laser pointer was chosen as a light source for the determination of tetracycline hydrochloride (TCH) based on complex formation with Fe(III) which produced a red color solution. The optimization was studied such as the concentration of Fe(III) and pH. The proposed method presented linearity in the concentration range 1.15 x 104 - 5.74 x 10 mol L-1 with R2 0.9987. The limit of detection (LOD) was 1.44 x 10 mol L-' and the percentage relative standard deviation (%RSD) for repeatability and reproducibility were 1.30 and 3.26, respectively. The proposed spectrometer was applied to determine TCH in pharmaceuticals compared to commercial spectrophotometers; it was found that there were no significant differences between the results of both equipment with 95% confidence. The lab-made spectrometer was an uncomplicated invention and successfil application for the analysis of TCH.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2023

Modified: 2023-05-29

Issued: 2023-05-29

บทความ/Article

application/pdf

BibliograpyCitation : In Mae Fah Luang University. School of Science. The Pure and Applied Chemistry International Conference 2023 (PACCON 2023) (pp.36-40). Chiang Rai : Mae Fah Luang University

eng

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