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Nattaporn Klinon. Identification of Coffea Arabica varieties using simple sequence repeat (SSR) and start codon targeted (SCoT) markers. Master's Degree(Biological Sciences). Mae Fah Luang University. Learning Resources and Educational Media Center. : Mae Fah Luang University, 2022.
Identification of Coffea Arabica varieties using simple sequence repeat (SSR) and start codon targeted (SCoT) markers
Name: Nattaporn Klinon
keyword:
SCoT
Classification :.DDC: e-Theses
Abstract:
Coffea arabica or arabica coffee is a high-value commercial crop that is predominantly grown in northern part of Thailand. Since the first introduction in Thailand, there were some arabica coffee varieties that have been cross-bred with sother varieties. Due to analogous morphological features, the identification of C. arabica varieties requires efficient molecular tools. In this study, Simple Sequence Repeat (SSR) and Start Codon Targeted (SCoT) markers are used to distinguish 18 accessions of C. arabica and Coffae spp. Eleven pairs of SSR primers produced moderately informative results with an average of 96.67% polymorphism and a mean PIC of 0.34, whereas 30 SCoT primers produced highly informative results with an average of 88.04% polymorphism and a mean PIC of 0.42. UPGMA clustering and PCoA using SSR markers demonstrated no significant difference for identifying of C. arabica varieties. In contrast, cluster analysis and PCoA using SCoT polymorphism demonstrated that the dendrogram revealed the efficiency to identify the varieties of C. arabica. However, SSR markers could be utilized to classified of Coffea spp. Combination of polymorphism data from both markers has generated the dendrogram with greater classification in the variety level, which distinguished closely related varieties among coffee samples with 0.9825 of similarity coefficient. Moreover, an analysis of minimized test revealed that a set of primers for SCoT marker (SC2, SC3, SC4, SC17, SC21, SC22, SC24 and SC32) have a potential to identify C. arabica in the variety level. This study demonstrated that SCoT markers is a preferable molecular tool for identification of C. arabica varieties.
Mae Fah Luang University. Learning Resources and Educational Media Center
Address:
CHIANG RAI
Email:
library@mfu.ac.th
Name: Natsaran Saichana
Role:
Advisor
Created:
2022
Modified:
2023-05-23
Issued:
2023-05-23
วิทยานิพนธ์/Thesis
application/pdf
CallNumber:
e-Theses
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Biological Sciences
Grantor: Mae Fah Luang University
©copyrights Mae Fah Luang University
RightsAccess:
ใช้เวลา
0.018266 วินาที
Nattaporn Klinon
| Title | Contributor | Type |
|---|---|---|
| Identification of Coffea Arabica varieties using simple sequence repeat (SSR) and start codon targeted (SCoT) markers
มหาวิทยาลัยแม่ฟ้าหลวง Nattaporn Klinon | Natsaran Saichana | วิทยานิพนธ์/Thesis |
Natsaran Saichana
| Title | Creator | Type and Date Create |
|---|---|---|
| Taxonomy and phylogeny of microfungi on grasses
มหาวิทยาลัยแม่ฟ้าหลวง Natsaran Saichana | Goonasekara, Ishani Dilhara | วิทยานิพนธ์/Thesis |
| Taxonomy and phylogeny of selected families of sordariomycetes with enphasis on seeds and fruits
มหาวิทยาลัยแม่ฟ้าหลวง Natsaran Saichana | Arachchige, Rekhani Hansika Perera Godagama Vidana | วิทยานิพนธ์/Thesis |
| Identification of Coffea Arabica varieties using simple sequence repeat (SSR) and start codon targeted (SCoT) markers
มหาวิทยาลัยแม่ฟ้าหลวง Natsaran Saichana | Nattaporn Klinon | วิทยานิพนธ์/Thesis |
| Taxonomy of lichenicolous fungi with emphasis on Hengduan mountains in China
มหาวิทยาลัยแม่ฟ้าหลวง Natsaran Saichana | Qingfeng Meng | วิทยานิพนธ์/Thesis |