Shotgun proteomic analysis of saliva identifies potential biomarkers for asthma

Name: Orapan Poachanukoon

keyword: Asthma control

; Saliva

; Shortgun proteomics

; Gene ontology

; Salivary proteins biomarkers

; LC-MS/MS

; STITCH

Abstract: Objective: The aim of this study was to determine if there was an association between the salivary protein profile and the disease control in asthma. Methods: Thirty asthmatic patients and 11 healthy volunteers were participated in this study. Skin prick testing with six aeroallergen including house dust mites, cockroach, grass, cat, dog and mold was carried out in the study. Demographic data, comorbidities, treatment drugs and the level of asthma control were also assessed in the asthmatics. Salivary samples were collected from the three study groups and were combined to form a pooled sample. Salivary proteins were separated using 1D SDSPAGE. The separated proteins were subjected to LC-MS/MS. Then, the raw MS/MS data were analyzed using the differential analysis software (DeCyder MS) for protein quantitation. The analyzed MS/MS data were sent to identify by Mascot MS/MS ions search using NCBI protein database. The identified and quantified proteins were then analyzed using the JVenn, MEV, Panther and STITCH version 5. Gene ontology annotations were performed using Panther database and protein-chemical interactions were analyzed according to STITCH 5.0 database. Results: In this study, patients’ age were ranged from 3.6 to 63 years. Most of the patients were female (66.7%). Twenty-two (73%) of the asthmatic patients were positive to allergy skin testing. Fourteen patients were assessed for controlled asthma. Most of controlled asthmatics received inhaled corticosteroids as the controller medications. Shotgun proteomic indicated that 193 salivary proteins were expressed in both controlled and uncontrolled asthma. They were predicted to associate with many proteins involved in pathogenesis of asthma including IL-5, IL-6, MCP-1, VEGF, and Periostin and asthma medicines (Cromolyn, Nedocromil, and Theophylline). Nucleoside diphosphate kinase (NME1-NME2) was only found in controlled asthmatics whereas polycystic kidney and hepatic disease 1 (PKHD1)/ fibrocystin, zinc finger protein 263 (ZNF263), uncharacterized LOC101060047 (ENSG00000268865), desmoglein 2 (DSG2) and S100 calcium binding protein A2 (S100A2) were only found in uncontrolled asthma. Therefore, the six salivary proteins can be used as potential biomarkers for asthma and they may be used to distinguish between controlled and uncontrolled asthma. Conclusion: Our proteomics data showed that nucleoside diphosphate kinase (NME1-NME2), polycystic kidney and hepatic disease 1 (PKHD1)/ fibrocystin, zinc finger protein 263 (ZNF263), uncharacterized LOC101060047 (ENSG00000268865), desmoglein 2 and S100A2 were associated with the disease control in asthma

Thammasat University. Thammasat University Library

Address: BANGKOK

Email: preserv@tu.ac.th

Name: Sitthichai Khunthongkaew

Role: advisor

Name: Sittiruk Roytrakul

Role: co-advisor

Created: 2020

Modified: 2023-02-23

Issued: 2023-02-23

วิทยานิพนธ์/Thesis

application/pdf

DOI: 10.14457/TU.the.2020.1151

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Oral Health Science

Grantor: Thammasat University

©copyrights Thammasat University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	10664orapan.pdf	2.18 MB	1	2024-04-30 15:55:47

ใช้เวลา

0.027535 วินาที