Sacran-collagen complex for cells culture application

Name: Saranyoo Sornkamnerd

Organization : Japan Advanced Institute of Science and Technology. School of Materials Science

Name: Okeyoshi, Kosuke

Organization : Japan Advanced Institute of Science and Technology. School of Materials Science

Email : kaneko@jaist.ac.jp

keyword: Sacran-collagen

LCSH: Cell coculture

; Aphanothece sacrum

LCSH: Polysaccharides

LCSH: Scanning electron microscopy

Abstract: The polysaccharide, sacran, derived from Aphanothece sacrum are huge anionic polysaccharides with high molecular weight and negative charge potential wells. Sacran has high cation absorption capability however low concentration of cation. So, we focus on the complexation between sacran and collagen. Collagen is major of tissue structure composed of amino groups and can show positive charge. Our objective is to prepare biomaterial scaffolds for cell attachment in tissue engineering application. Sacran hydrogel was prepared by solvent casting method. This hydrogel was coated by collagen to improve biocompatibility. Complexation between collagen and sacran is very efficient because of electrostatic force attraction between sacran polyanion and positive charge on amino groups. Finally porous scaffold was successfully prepared in consequence of freeze-drying process of sacran hydrogel. The scanning electron microscopy technique showed the porous size ranged in 63.73±15.08 and 60.67±12.83 μm for pure sacran and collagen coated sacran hydrogels, respectively. The porosity was 82.25 ± 7.43 % for pure sacran and 85.16 ± 12.70 % for collagen coated sacran. These scaffolds showed good cellular compatibility by promoting the growth of fibroblasts L929 cells. The results support that sacran scaffolds were established as effective scaffolds for cell attachment in tissue engineering applications

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2016

Modified: 2023-01-06

Issued: 2023-01-06

บทความ/Article

application/pdf

BibliograpyCitation : In Chemical Society of Thailand under the Patronage of Professor Dr. HRH Princess Chulabhorn and Chulalongkorn University Faculty of science. Pure and Applied Chemistry International Conference (PACCON 2016) (pp.1330-1333). Bangkok : Chemical Society of Thailand under the Patronage of Professor Dr. HRH Princess Chulabhorn

eng

©copyrights King Mongkut's University of Technology North Bangkok

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	PACCON 2016pp.1330-1333.pdf	308.81 KB

ใช้เวลา

0.022182 วินาที