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Phijittra Umalee. Identification of tea varieties collected from northern of Thailand using DNA barcodes. Master's Degree(Biological Sciences). Mae Fah Luang University. Learning Resources and Educational Media Center. : Mae Fah Luang University, 2022.
Identification of tea varieties collected from northern of Thailand using DNA barcodes
Name: Phijittra Umalee
keyword:
C. sinensis
Classification :.DDC: e-Theses
MeSH:
Plant biotechnology
-- 292510
MeSH:
Plant genetics
-- 292511
LCSH:
Plant biotechnology
-- 292510
LCSH:
Plant genetics
-- 292511
Abstract:
Tea plant (Camellia sinensis (L.) O. Kuntze) is an economically important crop in Thailand. Their leaves are frequently used as the primary ingredients in a variety of tea preparation. There are no reports on barcode analysis of wild tea plants in Thailand. Thus, the aimed of this study was to create a DNA barcode of 135 wild tea plants using the matK, and psbA- trnH genes. Both DNA barcodes were 100% successfully amplified and sequenced. The matK gene showed a good result in identification efficiency, accurately demonstrating 100% identity to the expected species, C. sinensis in 64 tea plants. Some tea plants displayed 100% identification with the other Camellia species, whereas the remaining tea plants displayed 99.10% to 99.89% identity with C. sinensis. In psbA-trnH just only one tea plant was detected 100% identity to C. sinensis and the remaining showed 97.24%-99.80%. Based on the blast results, all of the tea plants in this study were identified as C. sinensis. According to sequence alignment, matK detected 12 SNPs in 72 tea plants, which is more than psbA-trnH did (7 SNPS in 72 tea plants). The psbA-trnH showed the highest genetic distance (0.0131), which might be caused by indels. Phylogenetic trees clearly separated all wild tea plants into numerous groups. This demonstrated that tea plant with the same SNP position and pattern of SNPs are likely to be classified into the same cluster, though this was not entirely consistent with the morphological tree. As a results, DNA barcoding is a practical technique for identifying plant species, and the matK gene was thought to be a barcode for wild tea plants.
Mae Fah Luang University. Learning Resources and Educational Media Center
Address:
CHIANG RAI
Email:
library@mfu.ac.th
Name: Somrudee Nilthong
Role:
Advisor
Created:
2022
Modified:
2022-10-20
Issued:
2022-10-20
วิทยานิพนธ์/Thesis
application/pdf
CallNumber:
e-Theses
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Biological Sciences
Grantor: Mae Fah Luang University
©copyrights Mae Fah Luang University
RightsAccess:
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Phijittra Umalee
| Title | Contributor | Type |
|---|---|---|
| Identification of tea varieties collected from northern of Thailand using DNA barcodes
มหาวิทยาลัยแม่ฟ้าหลวง Phijittra Umalee | Somrudee Nilthong | วิทยานิพนธ์/Thesis |
Somrudee Nilthong
| Title | Creator | Type and Date Create |
|---|---|---|
| Local adaptations and vulnerabilities of mountain communities to climate change in Yunnan, China
มหาวิทยาลัยแม่ฟ้าหลวง Somrudee Nilthong, Ph. D.;Assoc. Prof. Kevin D. Hyde, Ph. D. | Su, Yufang | วิทยานิพนธ์/Thesis |
| Taxonomy and phylogeny of microfungi with emphasis on Vitis species
มหาวิทยาลัยแม่ฟ้าหลวง Assoc. Prof. Kevin David Hyde, Ph. D.;Somrudee Nilthong, Ph. D. | Jayawardena, Ruvishika S. | วิทยานิพนธ์/Thesis |
| Biology, phylogeny and taxonomy of fungicolous fungi in China and Thailand
มหาวิทยาลัยแม่ฟ้าหลวง Somrudee Nilthong;Kevin D. Hyde | Sun, Jingzu | วิทยานิพนธ์/Thesis |
| Genotypic screening of brown planthopper (Bph) resistance genes and evaluation of BPH resistance in upland rce varieties
มหาวิทยาลัยแม่ฟ้าหลวง Somrudee Nilthong | Kittiya Kanngan | วิทยานิพนธ์/Thesis |
| Identification of tea varieties collected from northern of Thailand using DNA barcodes
มหาวิทยาลัยแม่ฟ้าหลวง Somrudee Nilthong | Phijittra Umalee | วิทยานิพนธ์/Thesis |