The comparison between total phenolic content and antioxidant activity of gyokuro, sencha and matcha by using folin-ciocalteu and dpph method

Name: Prapaipun Piboolpunthuwong

keyword: Camellia sinensis

MeSH: Phenolic -- 288628

Classification :.DDC: e-Theses

; Total Phenolic Content, Antioxidant Activity, DPPH

MeSH: Antioxidants -- 288629

MeSH: Free radicals (Chemistry) -- 288630

LCSH: Phenolic -- 288628

LCSH: Antioxidants -- 288629

LCSH: Free radicals (Chemistry) -- 288630

Abstract: The objective of this study is to compare the total phenolic content and antioxidant activity between gyokuro, sencha and matcha from Chiang Rai and Bangkok. This research is very helpful for customers to choose the best green tea based on scientific studies. The result, sencha samples from Bangkok noted that sencha contains the most total phenolic content (179.074148 ± 1.154111 mgGAE/1g extract, followed by gyokuro (91.637316 ± 0.153648 mgGAE/1g extract) and matcha (78.510802 ± 0.450033 mgGAE/1g extract) respectively, and sencha is shown to have the highest antioxidant activity (IC50 equal to 0.007767 ± 0.000153 mg/ml), followed by gyokuro (IC50 0.013933 ± 0.000666 mg/ml) and matcha (IC50 0.019067 ± 0.000651 mg/ml) respectively. Samples from Chiang Rai noted that sencha contains the most total phenolic content (105.557527 ± 0.307296 mgGAE/1g extract), followed by matcha (101.080266 ± 0.122107 mgGAE/1g extract) and gyokuro (72.914226 ± 0.070499 mgGAE/1g extract) respectively. Sencha has the highest antioxidant activity (50% inhibitory concentration (IC50) equal to 0.008367 ± 0.000115 mg/ml) followed by matcha (IC50 is equal to 0.0016800 ± 0.000557 mg/ml) and gyokuro (IC50 is equal to 0.023267 ± 0.000503 mg/ml) respectively. According to the amount of total phenolic content and antioxidant activity, both factors can make the conclusion which kind of green tea is the best to drink. It noted that “Sencha” is the best green tea to drink because sencha samples from both Chiang Rai and Bangkok shows the same result. Most green tea samples contain higher antioxidant activity than the BHT standard, especially the sencha sample from Bangkok has higher antioxidant activity than the BHT standard by around 2-folds. Many factors are involved in the amount of total phenolic content and antioxidant activity such as sunlight, storage duration, pH value, oxygen, temperature, ingredients in products etc.

Mae Fah Luang University. Learning Resources and Educational Media Center

Address: CHIANG RAI

Email: library@mfu.ac.th

Name: Vitoon Jularattanaporn

Role: Advisor

Created: 2021

Modified: 2022-07-08

Issued: 2022-07-08

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: e-Theses

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Anti-Aging and Regenerative Medicine

Grantor: Mae Fah Luang University

©copyrights Mae Fah Luang University

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