Anti-inflammatory activity of some seaweed extracts from sattahip, Chonburi province

Name: Sirintip Pechroj

LCSH: Burapha University.Faculty of Science

Classification :.DDC: 579.1

LCSH: Marine Algae

Abstract: Seaweeds are an alternative source of bioactive substances for human health. They exhibit numerous biological activities including antioxidant and antiinflammatory activities. However, reports on the biological activities of seaweed harvested from the east coast of the Gulf of Thailand are limited. In this study, three types of brown seaweed, Dictyota cervicornis, Sargassum polycystum and Padina australis as well as green seaweed, Caulerpa lentillifera were collected in from Sattahip District, Chon Buri Province. The ethanol extracts of all four seaweeds were evaluated their intracellular antioxidant and anti-inflammatory activities by assessing inhibitory activity on the production of reactive oxygen species (ROS) and nitric oxide (NO) in lipopolysaccharide (LPS)-activated RAW264.7 macrophage cells using fluorescent probe H2DCF-DA and Griess reaction, respectively. Moreover, cell viability was studied by MTT assay. The results showed that P. australis, D. cervicornis, C. lentillifera and S. polycystum significantly inhibited both ROS and NO secretions in a concentration dependent manner without a significant cytotoxicity. ROS and NO productions were the most downgraded by D. cervicornis extract with the lowest IC50 for ROS (36.72 ± 1.53 µg/mL) and NO (19.97 ± 1.48 µg/mL), respectively. Therefore, it was selected to investigate the mechanism underlying the anti-inflammatory activity. The secretion of an another pro-inflammatory mediator, prostaglandin E2 (PGE2) was determined by ELISA Kit. D. cervicornis significantly inhibited the secretion of PGE2 into culture medium. The protein expression of inducible nitric oxide (iNOS) and cyclooxygenase-2 (COX-2), as well as inflammatory responsive proteins were determined by Western blot analysis. Also, mRNA expression of inflammatory genes was examined by real time RT-PCR technique. The results showed that D. cervicornis significantly reduced the mRNA expression of iNOS, COX-2, TNF-α, and IL-1β. Furthermore, D. cervicornis inhibited nuclear translocation of nuclear factor- κB (NF-κB) p65 subunit by decreased the phosphorylation of IκBα. It also suppressed the phosphorylation of three main mitogen activated protein kinases (MAPKs); ERK1/2, JNK and p38. Altogether, these data exhibited that D. cervivornis represses the production of pro-inflammatory mediators and cytokines via inactivation of NF-κB and MAPKs signaling pathways in LPS-induced RAW264.7 macrophages. Since, D. cervocornis seaweed extract exhibited intracellular antioxidant and anti-inflammatory activities, it might be used as a food ingredient for preventing oxidative stress and inflammation related diseases.

Burapha University. Library

Address: CHONBURI

Email: buulibrary@buu.ac.th

Name: Klaokwan Srisook

Role: Principal advisor

Name: Jariyavadee Suriyaphan

Role: Co-advisor

Created: 2020

Modified: 2022-05-09

Issued: 2022-05-09

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: Th 579.1 Si619A

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biochemistry

Grantor: Burapha University

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