Protein adsorption on electrospun nylon-6 and nylon-6/chitosan nanofibrous membrane

การดูดซับโปรตีนในน้ำโดยใช้เส้นใยนาโนของไนลอน 6 และ ไนลอน 6 ผสมไคโตซานที่ผลิตด้วยวิธีอิเล็กโตรสปินนิง

Name: Ratchanee Mongkollap

keyword: Protein

; Adsorption

; Nylon-6/chitosan blend

; Nanofibrous membrane

Abstract: Electrospun nylon-6 and nylon-6/chitosan nanofibrous membranes were successfully prepared by electrospinning technique. Electrospun nanofiber membranes were fabricated by varying nylon-6 concentrations of 25, 28 and 30% wt/v and the blends solutions were prepared by varying chitosan content in nylon-6 solution at the ratio of 100/2(NCS2), 100/4(NCS4) and 100/6(NCS6). Morphologies and the diameter distributions of nanofiber membranes were characterized by scanning electron microscopy (SEM) and an image analyzer program, respectively. The pore size of membrane was determined both by Hagen-Poiseuille and SEM image. The results showed that the solution with higher polymer concentration resulted in larger fiber diameters and larger pores. However, the ultrafine fibers along with large nylon-6/chitosan fibers were obtained from the blend solutions which resulted in smaller average fiber and pore sizes. These membranes were investigated to be used as adsorption medium for protein adsorption. Protein adsorption was done by flowing BSA solution through the membranes by syringe pump. The membrane thickness and BSA concentration were varied in this experiment. After adsorption process, BSA was leached from the membranes by either flowing pure water through the menbranes with the same flow rate of 8 µl/s or immersing the membranes in pure water at room temperature for 24 h. The remaining BSA concentration in the solution was determined by Bradford micro-assay. UV-VIS spectrophotometer was applied at a wavelength of 595 nm. The results revealed that the adsorption ability of both nylon-6 and composite nanofiber membranes increased with increasing membrane thickness, BSA concentration and chitosan content. Moreover, the results showed that the concentration of proteins in the leachate from nanofiber membranes by flowing water through the membranes was higher than that by immersing the membranes in water. The leached proteins on nylon membrane could also be removed more easily than that of composite membranes, confirming the strong interaction between chitosan functional groups and BSA. For dynamic adsorption, the study revealed that the adsorption mechanism composed of attachment and detachment of proteins on the surfaces. In addition, it was found that smaller pore size, larger surface area as well as more functional group of membrane could improve the adsorption ability of protein. In this work, N25CS6 membrane gave the highest adsorption efficiency with 68.19% which was higher than that of commercial membrane with 10.39% when 20 ppm-BSA concentration was used. This confirmed that electrospun nanofibrous membranes have a potential to be used for many application

Thammasat University. Thammasat University Library

Address: BANGKOK

Email: preserv@tu.ac.th

Name: Panu Danwanichakul

Role: advisor

Created: 2014

Modified: 2022-01-05

Issued: 2022-01-05

วิทยานิพนธ์/Thesis

application/pdf

DOI: 10.14457/TU.the.2014.448

eng

DegreeName: Master of Engineering

Level: Master's Degree

Descipline: Chemical Engineering

Grantor: Thammasat University

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