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Nakorn Sornsomboon. Development of colorimetric detection for genetically modified materials by using gold nanoparticles. Master's Degree(Biotechnology). Thammasat University. Thammasat University Library. : Thammasat University, 2015.
Development of colorimetric detection for genetically modified materials by using gold nanoparticles
Name: Nakorn Sornsomboon
keyword:
GM plants
Abstract:
Genetically modified plants (GM plants) are developed and wildly cultivated for supporting world food insufficient situation. Maize and soybean are the emphasized industrial plants that have been modifying their genetic for better cultivation. The negative feedbacks of GM plants make the trading conditions of GM plants and their products in some countries especially in EU or the other important countries for our imports. Therefore, in this research, the aim is to investigate colorimetric DNA sensor for pre-screening GM plants and their products by using gold nanoparticles (AuNPs) in order to achieve quickly detection. AuNPs was synthesized by citrate reduction method, it was around 18-20 nanometer in size. This sensing used sodium chloride (NaCl) and DNA probes system to complete the detection. Optimal of NaCl and DNA probe final concentration were 0.05 M and 4 µM, respectively. 8 of 15 samples from maize and soybean had positive results from 35S promoter and NOS terminator screening tests by polymerase chain reaction (PCR) method. Limit detection of PCR method was 0.01 ng. Colorimetric DNA sensor of this research had a specificity and fast detection time. The linear range of 35S detection was 1-30 µM and NOS detection was 10-50 µM. limit of detection (LOD) of 35S detection was 0.05 µM and NOS detection was 0.5 µM. Reproducibility of both detections were at 30 µM. Impurity of extracted DNA could be the detection interference, Taq DNA polymerase from PCR product was also included. Taq DNA polymerase at 0.025 UNIT and bovine serum albumin (BSA) at 200 µg/ml could aggregate AuNPs in detection system of this research
Thammasat University. Thammasat University Library
Address:
BANGKOK
Email:
preserv@tu.ac.th
Name: Theerachai Thanananta
Role:
advisor
Name: Pariya Na Nakorn
Role:
co-advisor
Created:
2015
Modified:
2021-11-29
Issued:
2021-11-29
วิทยานิพนธ์/Thesis
application/pdf
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Biotechnology
Grantor: Thammasat University
©copyrights Thammasat University
RightsAccess:
ใช้เวลา
0.030083 วินาที
Nakorn Sornsomboon
| Title | Contributor | Type |
|---|---|---|
| Development of colorimetric detection for genetically modified materials by using gold nanoparticles
มหาวิทยาลัยธรรมศาสตร์ Nakorn Sornsomboon | Theerachai Thanananta Pariya Na Nakorn | วิทยานิพนธ์/Thesis |
Theerachai Thanananta
| Title | Creator | Type and Date Create |
|---|---|---|
| Development of colorimetric detection for genetically modified materials by using gold nanoparticles
มหาวิทยาลัยธรรมศาสตร์ Theerachai Thanananta;Pariya Na Nakorn | Nakorn Sornsomboon | วิทยานิพนธ์/Thesis |
| Identification of genes involved in spikelet fertility in rice using RNA sequencing
มหาวิทยาลัยธรรมศาสตร์ Theerachai Thanananta;Amorntip Muangprom | Kanokwan Kaewmungkun | วิทยานิพนธ์/Thesis |
| Transcriptome analysis of rice seedling under cold stress using RNA sequencing
มหาวิทยาลัยธรรมศาสตร์ Theerachai Thanananta;Amorntip Muangprom | Patcharaporn Summat | วิทยานิพนธ์/Thesis |
Pariya Na Nakorn