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Ian Jones. Development of drop test immunoassay for melatonin detection. (). Mae Fah Luang University. Learning Resources and Educational Media Center. : , .
Development of drop test immunoassay for melatonin detection
Name: Pongsathon Phapugrangkul
Address:
333 Moo1, Thasud, Muang, Chiang Rai 57100
Organization :
School of Agro-Industry, Mae Fah Luang University
Email :
ask.library@mfu.ac.th
Name: Yuwada Rungrote
Name: Noppawan Dachraksa
Name: Ghit Laungsopapun
Name: Amorn owatworakit
Name: Siwatt Thaiudom
Name: Ian Jones
ThaSH:
Food sciences
ThaSH:
Agricultural industries
ThaSH:
Liquid chromatography
ThaSH:
Melatonin
ThaSH:
Melatonin - Detection
Abstract:
Melatonin (N-acetyl-5methoxytryptamine) plays an important role in the regulation of sleep cycles, immune functions and antioxidant capabilities. Various methods are used to determine melatonin concentration such as high-performance liquid chromatography (HPLC). However, this techniques is high-cost and not suitable for analyzing colloid samples such as milk. Enzyme-linked immunosorbent assay (ELISA) can be used for colloid samples but this is also expensive and time consuming. Thus, a fast and reliable method for melatonin detection was developed. Immunogenic melatonin was prepared by coupling melatonin with carrier protein as bovine serum albumin (BSA) using the Mannich reaction. After covalently bound with BSA, the melatonin-BSA coupling (Mel-BSA) was then used for rabbit immunization. The purified antibody was selected to construct a drop test immunoassay for melatonin detection. Under optimized condition, the limit of detection was shown to be 0.15 ng/mL with a turnaround time of about 20 minutes. This drop test immunoassay is a promising approach for development of a more rapid, robust and cost-effective tool for melatonin detection.
Mae Fah Luang University. Learning Resources and Educational Media Center
Address:
CHIANG RAI
Email:
library@mfu.ac.th
Modified:
2021-10-15
Issued:
2021-10-15
บทความ/Article
application/pdf
Journal of Food Science and Agricultural Technology (2019) Vol.5 (Spcl. Iss.): 229-233
eng
©copyrights Mae Fah Luang University
RightsAccess:
ใช้เวลา
-0.972875 วินาที
Pongsathon Phapugrangkul
| Title | Contributor | Type |
|---|---|---|
| Development of drop test immunoassay for melatonin detection
มหาวิทยาลัยแม่ฟ้าหลวง Pongsathon Phapugrangkul;Yuwada Rungrote;Noppawan Dachraksa;Ghit Laungsopapun;Amorn owatworakit;Siwatt Thaiudom;Ian Jones | บทความ/Article |
Yuwada Rungrote
| Title | Contributor | Type |
|---|---|---|
| Effect of cooking process on phytochemical contents and anticarcinogenic potential of Jasmine rice and Kum Doisaket rice
มหาวิทยาลัยเชียงใหม่ Yuwada Rungrote | Arunporn Itharat Rawinwan Wongpoomchai Klintean Wunnapuk | วิทยานิพนธ์/Thesis |
| Development of drop test immunoassay for melatonin detection
มหาวิทยาลัยแม่ฟ้าหลวง Pongsathon Phapugrangkul;Yuwada Rungrote;Noppawan Dachraksa;Ghit Laungsopapun;Amorn owatworakit;Siwatt Thaiudom;Ian Jones | บทความ/Article |
Noppawan Dachraksa
| Title | Contributor | Type |
|---|---|---|
| Development of drop test immunoassay for melatonin detection
มหาวิทยาลัยแม่ฟ้าหลวง Pongsathon Phapugrangkul;Yuwada Rungrote;Noppawan Dachraksa;Ghit Laungsopapun;Amorn owatworakit;Siwatt Thaiudom;Ian Jones | บทความ/Article |
Ghit Laungsopapun
| Title | Contributor | Type |
|---|---|---|
| Development of drop test immunoassay for melatonin detection
มหาวิทยาลัยแม่ฟ้าหลวง Pongsathon Phapugrangkul;Yuwada Rungrote;Noppawan Dachraksa;Ghit Laungsopapun;Amorn owatworakit;Siwatt Thaiudom;Ian Jones | บทความ/Article | |
| Green synthesis of titanium dioxide/ zinc oxide with Centella Asiatica (L.) extract for enhancing photocatalytic activity degradation of methylene blue
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Kittirat Prangchan;Khanittha Chawananorasest;Chaweewan Poonthananiwatkul;Ghit Laungsopapun;Singto Sakulkhaemaruethai | บทความ/Article |
Amorn owatworakit
| Title | Contributor | Type |
|---|---|---|
| Development of drop test immunoassay for melatonin detection
มหาวิทยาลัยแม่ฟ้าหลวง Pongsathon Phapugrangkul;Yuwada Rungrote;Noppawan Dachraksa;Ghit Laungsopapun;Amorn owatworakit;Siwatt Thaiudom;Ian Jones | บทความ/Article |
Siwatt Thaiudom
| Title | Contributor | Type |
|---|---|---|
| Development of drop test immunoassay for melatonin detection
มหาวิทยาลัยแม่ฟ้าหลวง Pongsathon Phapugrangkul;Yuwada Rungrote;Noppawan Dachraksa;Ghit Laungsopapun;Amorn owatworakit;Siwatt Thaiudom;Ian Jones | บทความ/Article |
Ian Jones
| Title | Contributor | Type |
|---|---|---|
| Development of drop test immunoassay for melatonin detection
มหาวิทยาลัยแม่ฟ้าหลวง Pongsathon Phapugrangkul;Yuwada Rungrote;Noppawan Dachraksa;Ghit Laungsopapun;Amorn owatworakit;Siwatt Thaiudom;Ian Jones | บทความ/Article |