Thickness and uniformity of spin coating poly (N-isopropylacrylamide-co-acrylamide) grafted culture sureace for cell sheet engineering

Name: Phongphot Sakulaue

keyword: Temperature responsive polymer

; Poly (N-isopropylacrylamide-co-acrylamide)

; UV grafting

; Thickness measurement

; Cell sheet engineering

; Polystyrene

; Thickness measurement

; Polymers

; PNIAM-co-AM

; Ellipsometry

Abstract: Poly (N-isopropylacrylamide-co-acrylamide) (PNIAM-co-AM) is known as temperature-responsive polymer that is used for collecting the cell sheet by using its phase transition property instead of using enzymes. In this study, PNIAM-co-AM is grafted onto tissue culture polystyrene surface (TCPS). Thermal polymerization and UV irradiation are developed in order to optimize a technique for preparation process. Other techniques such as evaporation method and spin-coating method are also examined for improving the preparation process. The uniformity of surface and thickness on copolymer layer are determined to improve the ability of cell sheet detachment and to make the process repeatable with reliable results. The physical properties of the grafted surfaces are confirmed by Fourier Transform Infrared Spectroscopy (FTIR), contact angle measurement and Atomic Force Microscopy (AFM). FTIR is used to observe the secondary amide group at 1650 cm-1. Contact angle measurement results show that the hydrophobic to hydrophilic transition is found in spin-coated copolymer dish. The surface hydrophilicity of spin-coated sample is higher than that of the samples prepared by other conditions. The topography of spin-coated copolymer shows flatter surface in contrast with ungrafted PS. It can confirm the uniform PNIAM grafted layer on substrate. Ellipsometry is used to derive models of both polystyrene and PNIAM-co-AM layer. From thickness measurement, spin-coated PNIAM-co-AM grafted PS samples obtain the thickness less than 30 nm. PNIAM-co-AM grafted PS samples are tested with mouse preosteoblast MC3T3-E1 cells. The results show that spin-coated copolymer is the most suitable surface for cell adhesion and detachment due to its uniform surface with optimum thickness

Thammasat University. Thammasat University Library

Address: BANGKOK

Email: preserv@tu.ac.th

Name: Wanwipa Siriwatwechakul

Role: advisor

Created: 2015

Modified: 2021-10-01

Issued: 2021-10-01

วิทยานิพนธ์/Thesis

application/pdf

DOI: 10.14457/TU.the.2015.50

eng

DegreeName: Master of Engineering

Level: Master's Degree

Descipline: Engineering Technology

Grantor: Thammasat University

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