Thai rice bran lipase-catalysed polyesterification : a development for practical applications

การใช้ไลเปสจากรำข้าวไทยในการสังเคราะห์พอลิเอสเทอร์ : การพัฒนาเพื่อใช้ในภาคปฏิบัติ

Name: Nonsee Nimitsiriwat

ThaSH: Rice bran

ThaSH: Lipase

ThaSH: Polyesters

Abstract: Two systems of Thai rice bran lipase (Thai RBL)-catalysed aliphatic polyester synthesis in toluene, i.e., adipic acid and 1,4-butanediol, and adipic acid and poly(ethylene glycol) MW. 200, were earned out at stoichiometric ratio. The degree of polymerization was found to be 4 and 9 in the former case and 2 and 6 in the latter case. The polymerization was accelerated by the reaction temperature at 80⁰ to 100 ℃ and long reaction time for over 7 days. Thai RBL was immobilized via physisorption and covalent bonding to improve enzyme activity. The physisorption of Thai RBL onto fumed silica was optimized at 1:40 for carrier:crude enzyme (g/mL) to give 38% immobilization with hydrolytic activity of 21 mU/mg. The covalent bonding of Thai RBL onto fumed silica was achieved at 50% immobilization with hydrolytic activity of 9 mU/mg. The utilization of purified enzyme to immobilize by physisorption and covalent bonding improved the hydrolytic activity for three times comparing to that of crude enzyme.

Abstract: Two systems of Thai rice bran lipase (Thai RBL)-catalysed aliphatic polyester synthesis in toluene, i.e., adipic acid and 1,4-butanediol, and adipic acid and poly(ethylene glycol) MW. 200, were earned out at stoichiometric ratio. The degree of polymerization was found to be 4 and 9 in the former case and 2 and 6 in the latter case. The polymerization was accelerated by the reaction temperature at 80⁰ to 100 ℃ and long reaction time for over 7 days. Thai RBL was immobilized via physisorption and covalent bonding to improve enzyme activity. The physisorption of Thai RBL onto fumed silica was optimized at 1:40 for carrier:crude enzyme (g/mL) to give 38% immobilization with hydrolytic activity of 21 mU/mg. The covalent bonding of Thai RBL onto fumed silica was achieved at 50% immobilization with hydrolytic activity of 9 mU/mg. The utilization of purified enzyme to immobilize by physisorption and covalent bonding improved the hydrolytic activity for three times comparing to that of crude enzyme.

Chulalongkorn University. Office of Academic Resources

Address: BANGKOK

Email: cuir@car.chula.ac.th

Name: Suwabun Chirachanchai

Role: Advisor

Name: Rath Pichyangkura

Role: Advisor

Name: Ratana Rujiravanit

Role: Advisor

Name: Erdogan,Gulari

Role: Advisor

Created: 2000

Modified: 2564-09-09

Issued: 2021-08-23

วิทยานิพนธ์/Thesis

application/pdf

URL: http://cuir.car.chula.ac.th/handle/123456789/72556

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Polymer Science

Grantor: Chulalongkorn University

©copyrights Chulalongkorn University

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