Protocol development in sampling foliicolous lichens in varying leaf areas

Name: Paguirigan JAG

Address: 333 Moo1, Thasud, Muang, Chiang Rai 57100

Organization : Center of Excellence in Fungal Research, Mae Fah Luang University

Email : ask.library@mfu.ac.th

Name: Chan AB

Name: Villanueva CKG

Name: Bagaoisan JB

Name: Matalicia SJB

keyword: leaves – lichen – linear regression – Mount Isarog – Protocol

ThaSH: Fungi

ThaSH: Leaves

ThaSH: Lichens

Abstract: The Philippines, a biodiversity hotspot, harbors various types of flora and fauna and yet its biodiversity is heavily threatened. The country is home to some of the richest and most important ecosystems which house a high percentage of plant and animal life that are vulnerable to extinction. With this, the great interest of research in the country focuses on biodiversity. However, limited information on the Philippine lichen flora is available and foliicolous lichens are still untapped and have long remained unknown. To understand more about these organisms, 140 leaf samples were obtained from 14 host plants collected at Consocep, Mount Isarog, Tigaon, Camarines Sur, Philippines in this study. The mean percentage of foliicolous lichen area compared to the leaf area observed as a whole, by half, and by quarter was analyzed using the analysis of variance (ANOVA). Means and its standard errors (SEM) were used to summarize the percentage of leaf area with foliicolous lichen. The results showed that the foliicolous lichen area measured for the whole, half or quarter leaf areas gave the same data and analysis results, when the leaf age is considered. Linear regression model also showed the positive correlation between the lichen area and the leaf area. A protocol was made for the first time to analyze the distribution of foliicolous lichens on various leaf samples. The developed protocol in studying foliicolous lichens includes (1) the use of mature leaves (9th–13th leaf from the apical bud), (2) use of half or quarter portion of the leaf for longer, wider leaves, (3) use of a 1 cm × 1 cm transparent leaf grid to measure the leaf area, and (4) the use of regression model to compute the area covered by the foliicolous lichens.

Mae Fah Luang University. Learning Resources and Educational Media Center

Address: CHIANG RAI

Email: library@mfu.ac.th

Modified: 2021-08-20

Issued: 2021-08-20

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BibliograpyCitation : Asian Journal of Mycology (AJOM). Vol.3, No.1 (January - June 2020): p.316-324

Asian Journal of Mycology 3(1): 316–324 (2020)

eng

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