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Saranya Sedtananun. Establishment of 3D non-small cell lung cancer spheroid as a pre-clinical model for anti-cancer effect assessment of the potential cationic antimicrobial peptide (Cn-AMP1) from coconut water. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2020.
Establishment of 3D non-small cell lung cancer spheroid as a pre-clinical model for anti-cancer effect assessment of the potential cationic antimicrobial peptide (Cn-AMP1) from coconut water
Name: Lueacha Tabtimmai
Organization :
King's Mongkut University of North Bangkok. Faculty of Applied Science
Name: Grabowska, Anna
Organization :
University of Nottingham. School of Medicine
Name: Kiattawee Choowongkomon
Organization :
Kasetsart University. Faculty of Science
Name: Sirikhwan Tinrat
Organization :
King's Mongkut University of North Bangkok. Faculty of Applied Science
Name: Saranya Sedtananun
Organization :
King's Mongkut University of North Bangkok. Faculty of Applied Science
Email :
saranya.s@sci.kmutnb.ac.th
keyword:
Spheroid
LCSH:
Cell culture
Abstract:
The 3D cellular spheroid model becomes a current cellular model for cytotoxicity assessment. According to its advantages, the model recapitulates cell-cell interaction related to physiological aspects in humans. Non-small cell lung cancer has been diagnosed as a major population of lung cancer patients. Herein, A549 cells were used as a cellular model for 3D spheroid establishment. The number of cells and culturing time were optimized to reach an optimal condition. 1x103 cells/well elicited the highest cell viability at day 5. In the presence of extracellular matrix, A549 spheroid slightly increased cell viability compared to ECM-deprived conditions. Immunofluorescent staining also revealed proliferative cells at the peripheral rim (Calcein-AM stained), whereas necrotic cells gradually increased over time at the core of the spheroid in both conditions. For drug assessment, CnAMP1 was previously reported to be a promising peptide against colorectal cancer. As anticipated, Cn-AMP1 decreased the cell viability of A549 in a dose-dependent manner. In parallel, 3D A549 spheroids were set up for cytotoxicity assay. Surprisingly, Cn-AMP1 did not demonstrate any effect against the model. Taken together, the 3D spheroid model might be feasible to perform as a preclinical model for cytotoxicity assessment leading to reduce the use of animals in research
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2020
Modified:
2021-05-28
Issued:
2021-05-28
บทความ/Article
application/pdf
BibliograpyCitation :
In Thai Society for Biotechnology. The 32nd Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2020) (pp.701-712). Bangkok : The Thai Society for Biotechnology
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.038882 วินาที
Lueacha Tabtimmai
| Title | Contributor | Type |
|---|---|---|
| Establishment of 3D non-small cell lung cancer spheroid as a pre-clinical model for anti-cancer effect assessment of the potential cationic antimicrobial peptide (Cn-AMP1) from coconut water
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Lueacha Tabtimmai;Grabowska, Anna;Kiattawee Choowongkomon;Sirikhwan Tinrat;Saranya Sedtananun | บทความ/Article | |
| Aptamer-based inhibitor screening against the tyrosine kinase domain of EGFR
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Chakpetch Kuitio;Phongphat Obunchoey;Lueacha Tabtimmai;Sasimanas Unajak;Kiattawee Choowongkomon | บทความ/Article | |
| Parameterization on fructose-stabilized silver nanoparticle synthesis by non-thermal atmospheric pressure helium plasma jet
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Jirapong Sornsakdanuphap.;Khuanjarat Choengpanya.;Chanthana Susawaengsup.;Lueacha Tabtimmai.;Kiattawee Choowongkomon. | บทความ/Article | |
| Secondary metabolites and cytotoxic effects of Butea monosperma flowers grown in Thailand on HeLa and HCT116 cancer cell lines
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Sutarin Preepram;Lueacha Tabtimmai;Kiattawee Choowongkomon;Prasat Kittakoop;Supachai Jadsadajerm;Awat Wisetsai | บทความ/Article |
Grabowska, Anna
| Title | Contributor | Type |
|---|---|---|
| Establishment of 3D non-small cell lung cancer spheroid as a pre-clinical model for anti-cancer effect assessment of the potential cationic antimicrobial peptide (Cn-AMP1) from coconut water
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Lueacha Tabtimmai;Grabowska, Anna;Kiattawee Choowongkomon;Sirikhwan Tinrat;Saranya Sedtananun | บทความ/Article |
Kiattawee Choowongkomon
| Title | Contributor | Type |
|---|---|---|
| Establishment of 3D non-small cell lung cancer spheroid as a pre-clinical model for anti-cancer effect assessment of the potential cationic antimicrobial peptide (Cn-AMP1) from coconut water
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Lueacha Tabtimmai;Grabowska, Anna;Kiattawee Choowongkomon;Sirikhwan Tinrat;Saranya Sedtananun | บทความ/Article | |
| Aptamer-based inhibitor screening against the tyrosine kinase domain of EGFR
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Chakpetch Kuitio;Phongphat Obunchoey;Lueacha Tabtimmai;Sasimanas Unajak;Kiattawee Choowongkomon | บทความ/Article | |
| Development of electrochemical aptasensor for detection of Escherichia coli in swine
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Siwaporn Prachoochote ;Charoenkwan Kraiya;Kiattawee Choowongkomon | บทความ/Article | |
| Development of aptamer-based detection kit by electrochemistry for rapid assessment of vitamin D levels
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Sattawat Boonchaleaw ;Supaphorn Seetaha;Kiattawee Choowongkomon | บทความ/Article | |
| Portable sensor for Dengue virus detection by modified screen-printed electrode
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Wattanachai Boonprapha;Supaphorn Seetaha;Kiattawee Choowongkomon;Chaiya Prasittichai | บทความ/Article | |
| Parameterization on fructose-stabilized silver nanoparticle synthesis by non-thermal atmospheric pressure helium plasma jet
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Jirapong Sornsakdanuphap.;Khuanjarat Choengpanya.;Chanthana Susawaengsup.;Lueacha Tabtimmai.;Kiattawee Choowongkomon. | บทความ/Article | |
| Secondary metabolites and cytotoxic effects of Butea monosperma flowers grown in Thailand on HeLa and HCT116 cancer cell lines
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Sutarin Preepram;Lueacha Tabtimmai;Kiattawee Choowongkomon;Prasat Kittakoop;Supachai Jadsadajerm;Awat Wisetsai | บทความ/Article |
Sirikhwan Tinrat
| Title | Contributor | Type |
|---|---|---|
| Phytochemical screenings and anti-vibrio parahaemolyticus activities of senna occidentalis Linn. leaves extracts
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Sirikhwan Tinrat;Chatchawan Singhapol;Saranya Sedtananun | บทความ/Article | |
| Establishment of 3D non-small cell lung cancer spheroid as a pre-clinical model for anti-cancer effect assessment of the potential cationic antimicrobial peptide (Cn-AMP1) from coconut water
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Lueacha Tabtimmai;Grabowska, Anna;Kiattawee Choowongkomon;Sirikhwan Tinrat;Saranya Sedtananun | บทความ/Article |
Saranya Sedtananun
| Title | Contributor | Type |
|---|---|---|
| Phytochemical screenings and anti-vibrio parahaemolyticus activities of senna occidentalis Linn. leaves extracts
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Sirikhwan Tinrat;Chatchawan Singhapol;Saranya Sedtananun | บทความ/Article | |
| Establishment of 3D non-small cell lung cancer spheroid as a pre-clinical model for anti-cancer effect assessment of the potential cationic antimicrobial peptide (Cn-AMP1) from coconut water
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Lueacha Tabtimmai;Grabowska, Anna;Kiattawee Choowongkomon;Sirikhwan Tinrat;Saranya Sedtananun | บทความ/Article |