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Soraya Chaturongakul. Construction of recombinant PWH1520 vector expressing double-stranded RNA targeting vp28 of white spot syndrome virus in E. Coli. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2020.
Construction of recombinant PWH1520 vector expressing double-stranded RNA targeting vp28 of white spot syndrome virus in E. Coli
Name: Kanokwan Dekham
Organization :
Mahidol University. Faculty of Science
Name: Sarocha Jitrakorn
Organization :
Mahidol University. Faculty of Science
Name: Patai Charoonnart
Organization :
Mahidol University. Faculty of Science
Name: Vanvimon Saksmerprome
Organization :
Mahidol University. Faculty of Science
Name: Soraya Chaturongakul
Organization :
Mahidol University. Faculty of Science
Email :
soraya.cha@mahidol.ac.th
keyword:
White spot syndrome virus
LCSH:
RNA
Abstract:
An envelope protein VP28 of white spot syndrome virus (WSSV), one of the most lethal viruses affecting Penaeus vannamei shrimp, serves as an excellent target to control WSSV through RNA interference (RNAi) mechanism. pWH1520 vector was selected to express dsRNA for propagation in Gram-negative and Gram-positive bacteria. Thus, the objective of this study was to engineer Escherichia coli expressing dsRNA targeting VP28 through pWH1520 expression vector. Recombinant pWH1520-hpVP28 was successfully constructed by restriction enzyme digestion and propagated in E. coli DH5a. Double stranded RNA targeting VP28 (dsVP28) was successfully produced by 0.25 percent xylose inducible hairpin expression vector (pWH1520-hpVP28) in RNaseIII deficient E. coli. Additionally, dsVP28 was confirmed by digestion dsVP28 with RNaseIII. Absence of dsVP28 was found in dsRNA digested condition with RNaseIII. We concluded that E. coli containing recombinant pWH1520-hpVP28 expression vector could produce dsVP28 and further serve as an VP28 RNAi delivery shuttle
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2020
Modified:
2021-05-17
Issued:
2021-05-17
บทความ/Article
application/pdf
BibliograpyCitation :
In Thai Society for Biotechnology. The 32nd Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2020) (pp.1-4). Bangkok : The Thai Society for Biotechnology
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.023611 วินาที
Kanokwan Dekham
| Title | Contributor | Type |
|---|---|---|
| Construction of recombinant PWH1520 vector expressing double-stranded RNA targeting vp28 of white spot syndrome virus in E. Coli
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Kanokwan Dekham;Sarocha Jitrakorn;Patai Charoonnart;Vanvimon Saksmerprome;Soraya Chaturongakul | บทความ/Article |
Sarocha Jitrakorn
| Title | Contributor | Type |
|---|---|---|
| Construction of recombinant PWH1520 vector expressing double-stranded RNA targeting vp28 of white spot syndrome virus in E. Coli
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Kanokwan Dekham;Sarocha Jitrakorn;Patai Charoonnart;Vanvimon Saksmerprome;Soraya Chaturongakul | บทความ/Article |
Patai Charoonnart
| Title | Contributor | Type |
|---|---|---|
| Construction of recombinant PWH1520 vector expressing double-stranded RNA targeting vp28 of white spot syndrome virus in E. Coli
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Kanokwan Dekham;Sarocha Jitrakorn;Patai Charoonnart;Vanvimon Saksmerprome;Soraya Chaturongakul | บทความ/Article |
Vanvimon Saksmerprome
| Title | Contributor | Type |
|---|---|---|
| Construction of recombinant PWH1520 vector expressing double-stranded RNA targeting vp28 of white spot syndrome virus in E. Coli
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Kanokwan Dekham;Sarocha Jitrakorn;Patai Charoonnart;Vanvimon Saksmerprome;Soraya Chaturongakul | บทความ/Article |
Soraya Chaturongakul
| Title | Contributor | Type |
|---|---|---|
| Construction of recombinant PWH1520 vector expressing double-stranded RNA targeting vp28 of white spot syndrome virus in E. Coli
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Kanokwan Dekham;Sarocha Jitrakorn;Patai Charoonnart;Vanvimon Saksmerprome;Soraya Chaturongakul | บทความ/Article |