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Usa Kritsadaruangchai. Solid state fermentation of by-products from assamtea leaves with trichoderma spp.. Master's Degree(Cosmetic Science). Mae Fah Luang University. Learning Resources and Educational Media Center. : Mae Fah Luang University, 2021.
Solid state fermentation of by-products from assamtea leaves with trichoderma spp.
Name: Usa Kritsadaruangchai
Organization :
Mae Fah Luang University
keyword:
Solid state
ThaSH:
Solid-state fermentation
-- Biotechnology
;
Tea
ThaSH:
Solid-state fermentation
-- Tea
Abstract:
Even though mature Assam tea leaves are generally considered as agricultural wastes, they are rich in many bioactive compounds. In this study, tea leaves were used as new substrate for solid state fermentation (SSF) by different isolates of Trichoderma including Trichoderma harzianum (T. harzianum) CB-Pin-01, Trichoderma sp. NTY211, Trichoderma sp. PSUT001 and Trichoderma sp. PSUT002. Based on morphological evidence, all isolates were closely related to T. harzianum. They were proven that they highly possessed cellulase activity, but low tannase activity was observed. This indicates their ability to degrade cellulose-based sources.
Mature Assam tea leaves were fermented with Trichoderma spp. by using SSF. In addition, changes in phenolic contents and antioxidant activity of the fermented Assam tea leaves extracts were also explored. It was conducted in Erlenmeyer flask by inoculating the spore suspension and incubated at 30°C for 7 days. Different solvents including water, 50% (v/v) ethanol, 50% (v/v) acetone, 95% (v/v) ethanol, and 95% (v/v) acetone were used to extract phenolic compounds. Total phenolic content (TPC), total flavonoid content (TFC), DPPH and ABTS radicals scavenging activities, and ferric reducing antioxidant power (FRAP) were evaluated. The results revealed that TPC, TFC, and antioxidant activity were decreased in all fermented extracts as compared to unfermented extracts. Evaluation of TPC, TFC and antioxidant activity demonstrated that fermentation of Assam tea leaves dramatically decreased TPC and TFC as well as antioxidant activity. The highest remained TPC (125.71±3.61 mg GAE/g extract) was obtained in the presence of 95% (w/w) acetone, whereas TFC was highly remained at 130.30±5.05 and 126.64±3.14 mg QE/g extract in the presence of 50% and 95% (v/v) acetone, respectively. The IC50 values assayed by DPPH and ABTS were remained at 27.98±0.17 μg/mL (50% acetone extract) and 11.05±0.11 μg/mL (95% acetone extract), respectively. Moreover, the highest FRAP value (506.58±26.49 mg TEAC/g extract) was represented in the 50% (v/v) acetone extract. This indicates that SSF by Trichoderma spp. affected the reduction of phenolic contents and antioxidants activity that could not be proper for bioactivity improvement of Assam tea leaves...
Mae Fah Luang University. Learning Resources and Educational Media Center
Address:
CHIANG RAI
Email:
library@mfu.ac.th
Name: Punyawatt Pintathong,
Role:
Advisor
Created:
2021
Modified:
2021-01-27
Issued:
2021-01-27
วิทยานิพนธ์/Thesis
application/pdf
CallNumber:
Thesis TP248.25.S64 U842s 2019
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Cosmetic Science
Grantor: Mae Fah Luang University
©copyrights Mae Fah Luang University
RightsAccess:
ใช้เวลา
0.030861 วินาที
Usa Kritsadaruangchai
| Title | Contributor | Type |
|---|---|---|
| Alteration of phenolic content and antioxidant capacity of mature Assam tea leaves by solid state fermentation using Trichoderma sp. isolate NTY211
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Usa Kritsadaruangchai;Phanuphong Chaiwut;Putarak Chomnunti;Warin Intana;Angkana Saikeur;Sarita Sangthong;Punyawatt Pintathong | บทความ/Article | |
| Solid state fermentation of by-products from assamtea leaves with trichoderma spp.
มหาวิทยาลัยแม่ฟ้าหลวง Usa Kritsadaruangchai | Punyawatt Pintathong, | วิทยานิพนธ์/Thesis |
| Effect of solid state fermentation with Trichoderma spp. on phenolic content and antioxidant capacities of mature Assam tea leaves
มหาวิทยาลัยแม่ฟ้าหลวง Usa Kritsadaruangchai;Phanuphong Chaiwut;Putarak Chomnunti;Narit Thaochan;Angkana Saikeur;Punyawatt Pintathong | บทความ/Article |
Punyawatt Pintathong,
| Title | Creator | Type and Date Create |
|---|---|---|
| Extraction of tyrosinase from edible mushrooms for applications in cosmetics
มหาวิทยาลัยแม่ฟ้าหลวง Phanuphong Chaiwut, Ph. D.;Punyawatt Pintathong, Ph. D. | Phapawee Ieamsakul | วิทยานิพนธ์/Thesis |
| Extraction and biological activities of water-soluble polysaccharides from edible mushrooms for cosmetic applications
มหาวิทยาลัยแม่ฟ้าหลวง Phanuphong Chaiwut, Ph. D.;Punyawatt Pintathong, Ph. D. | Patcharee Pongsua | วิทยานิพนธ์/Thesis |
| Solid state fermentation of by-products from assamtea leaves with trichoderma spp.
มหาวิทยาลัยแม่ฟ้าหลวง Punyawatt Pintathong, | Usa Kritsadaruangchai | วิทยานิพนธ์/Thesis |