Abstract:
This experiment was conducted on feeding the female black tiger shrimp (Penaeus
monodon) with four different diets. The control group of female shrimps was fed with the sand
polychaetes (Perinereis sp.) and the experimental groups of shrimps were fed with the
earthworms (African night crawler, Eudrilus eugeniae) supplemented with 17β-estradiol hormone
in three different concentration levels of 0, 500 and 1,000 μg/kg of bedding. Artificial
insemination and eggspawning for two times per black tiger shrimp broodstock were performed
tostudy on the effect of 17β-estradiol supplementation in African night crawler on female black
tiger shrimp broodstock maturation. This study was observed on the period of ovary development,
spawning rate, fertilization rate, hatching rate, survival rate and hormone levels in blood of the
black tiger shrimp broodstock. The results showed that the artificial insemination and egg
spawning of both times in ovarian development period of female shrimps fed with sand
polychaetes and earthworms supplemented with 17β-estradiol at 500 and 1,000 μg/kg of bedding
showed no significant difference (p>0.05) but significantly different (p<0.05) with the shrimp fed
with African earthworms without supplemented hormones on a slow ovarian development period.
There were no statistically significant differences in fertilization rate, hatching rate and survival
rate among the four groups (p>0.05). For the study on hormone level in shrimp blood, the female
shrimp broodstock had slightly increased in 17β-estradiol hormone when the shrimps were fed
withhigh level of the hormone supplemented in the earthworms. From the results, the
earthworms supplemented with 17β-estradiol hormone levels of 500 μg/kg of bedding was the
appropriate hormone level for the female black tiger shrimp broodstock maturation.