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Srimek Chowpongpang.. Identification of GST interacted proteins under PRSV infected papaya using affinity purification-mass spectrometry. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2020.
Identification of GST interacted proteins under PRSV infected papaya using affinity purification-mass spectrometry
Name: Aroonothai Sawwa.
Organization :
Kasetsart University Kampaeng Saen Campus. Center for Agricultural Biotechnology
Email :
aroonothai.s@doa.in.th
Name: Yodying Yingchutrakul.
Organization :
Thailand Science Park. National Center for Genetic Engineering and Biotechnology
Name: Sittiruk Roytrakul.
Organization :
Thailand Science Park. National Center for Genetic Engineering and Biotechnology
Name: Wanwisa Siriwan.
Organization :
Kasetsart University. Faculty of Agriculture
Name: Srimek Chowpongpang.
Organization :
Kasetsart University Kampaeng Saen Campus. Center for Agricultural Biotechnology
LCSH:
PAPAYA RINGSPOT VIRUS.
LCSH:
GLUTATHIONE TRANSFERASE.
Abstract:
Glutathione S-transferases (GSTs) are multifunctional proteins involved in stress metabolism, which play major roles in biotic and abiotic stress responses. GSTs have found in all organisms which is a major phase II detoxification enzymes found in the cytosol. GSTs regulate peroxidase and isomerase activities, they protect cells against H(2)O(2)-induced cell death. Papaya rigsport virus (PRSV) is one of main biotic agent that cause damage in papaya. The disease symptoms are mosaic, chrolosis, ring spot and stunt, all the symptoms are the early stage of cell death. The main point of this research to investigate protein interaction of the PRSV interacted plant proteins and GSTs recombinant protein using the classical Affinity-purification-mass spectrometry (AP-MS) approache. The GST protein was heterologous expressed in E.coli system and the pull down assay was applied to expore the protein interacted complexs after that the protein complexs were indentified by LC-MS/MS. CTC1, Protein CCA1 isoform X1, Tetratricopeptide repeat (TPR)-like superfamily protein, PHD finger protein ALFIN-LIKE 9 and Fructose-bisphosphate aldolase-lysine N-methyltransferase proteins were identified. The interacted five proteins were predicted the protein network by STITCH program, the result show they associated with oxidative stress response mechanism. However, this is the basic intensive information that could develop to manufacture PRSV resistance variety in the future.
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2020
Modified:
2563-08-11
Issued:
2020-08-11
บทความ/Article
application/pdf
BibliograpyCitation :
APPLIED SCIENCE AND ENGINEERING PROGRESS. vol. 13, no. 3 (July-Sept. 2020), p. 224-232.
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.042278 วินาที
Aroonothai Sawwa.
| Title | Contributor | Type |
|---|---|---|
| Identification of GST interacted proteins under PRSV infected papaya using affinity purification-mass spectrometry
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Aroonothai Sawwa.;Yodying Yingchutrakul.;Sittiruk Roytrakul.;Wanwisa Siriwan.;Srimek Chowpongpang. | บทความ/Article |
Yodying Yingchutrakul.
| Title | Contributor | Type |
|---|---|---|
| Identification of GST interacted proteins under PRSV infected papaya using affinity purification-mass spectrometry
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Aroonothai Sawwa.;Yodying Yingchutrakul.;Sittiruk Roytrakul.;Wanwisa Siriwan.;Srimek Chowpongpang. | บทความ/Article |
Sittiruk Roytrakul.
| Title | Contributor | Type |
|---|---|---|
| Shotgun proteomic analysis of germinated rice (Oryza sativa L) under salt stress
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Li, Rui.;Trilert Chaicherdsakul.;Viyada Kunathigan.;Sittiruk Roytrakul.;Atchara Paemanee.;Suthathip Kittisenachai. | บทความ/Article | |
| Identification of GST interacted proteins under PRSV infected papaya using affinity purification-mass spectrometry
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Aroonothai Sawwa.;Yodying Yingchutrakul.;Sittiruk Roytrakul.;Wanwisa Siriwan.;Srimek Chowpongpang. | บทความ/Article | |
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Wanwisa Siriwan.
| Title | Contributor | Type |
|---|---|---|
| Identification of GST interacted proteins under PRSV infected papaya using affinity purification-mass spectrometry
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Aroonothai Sawwa.;Yodying Yingchutrakul.;Sittiruk Roytrakul.;Wanwisa Siriwan.;Srimek Chowpongpang. | บทความ/Article |
Srimek Chowpongpang.
| Title | Contributor | Type |
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| Identification of GST interacted proteins under PRSV infected papaya using affinity purification-mass spectrometry
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Aroonothai Sawwa.;Yodying Yingchutrakul.;Sittiruk Roytrakul.;Wanwisa Siriwan.;Srimek Chowpongpang. | บทความ/Article |