Kamonwan Khanthong. Effect of extruded activated carbon on bioenergy production from shrimp pond sediment and changing of microbial diversity. Master's Degree(Bioengineering). Burapha University. Library. : Burapha University, 2017.
Effect of extruded activated carbon on bioenergy production from shrimp pond sediment and changing of microbial diversity
Abstract:
Renewable energy has been interested for replacing fossil fuels nowadays.
Biogas is one of promising renewable energy that can be produced during anaerobic
digestion process. Shrimp pond sediment is considering as biomass from an agriculture
waste in Thailand that had been reported its ability as feedstock for producing biogas as
well as using of immobilized media for microbial attachment to improve microbial
availability in biogas production. In this study, three different types of materials
including extruded activated carbon (EAC), extruded zeolite (EZ) and extruded
activated carbon mixed zeolite (EACZ) were investigated as supporter for microbial
attachment. In previous study, these materials were submersed in synthetic wastewater
that performed in semi-batch reactor for 14 days to investigate the COD removal and
biogas production. The analysis was continuing in this project. We found that EAC
showed as the most appropriated material due to highest number of colonies on material
surface from SEM analysis and DNA concentration. EAC was varied in three different
ratios, 3%, 4% and 5% w/w in purpose of yielding bioenergy production using shrimp
pond sediment as a substrate. The result showed that EAC digesters accumulated higher
H2 concentration than control reactor. Among EAC digesters, increasing of EAC amount
resulted in obtaining higher CH4 concentration. However, the control reactor showed
higher CH4 production compare to EAC digesters. For microbial diversity analysis,
predominant bacteria in shrimp pond sediment after biogas production was
Alcaligenaceae family which is identified as methane oxidizing bacteria. Moreover,
EAC could increase the number of archaea in the digesters. In term of environmental
issue, volume of shrimp sediment per ponds are average 220 m3 that contained 150 tons
of solid content. Application of anaerobic digestion for treating shrimp pond sediment
can approximately reduce 75 tons of solid content together with producing 113 m3 of
biogas per shrimp pond. So, water pollutant can be reduced together with producing
renewable energy in a sustainable way.