Effect of black rice bran and beta-glucan on prevention of colon cancer-related colitis in cats

ผลของรำข้าวดำและบีต้ากลูแคนต่อการป้องกันลำไส้อักเสบที่สัมพันธ์กับมะเร็งลำไส้ใหญ่ในหนูขาว

Name: Noppawat Pengkumsri

LCSH: Herbs -- Therapeutic use

Classification :.DDC: 615.321

LCSH: Rice bran

Abstract: The colorectal cancer is reported by World health Organization (WHO) that the incident of colorectal cancer is third most of all global cancer types and increasing every year. In Thailand, the National Cancer Institute reported that the incidence of colorectal cancer in the male is second most incident report followed by lung cancer, in the female is third most common followed breast cancer, and cervical cancer. The major causes are improper diet habits, and loss of immunity. Thus, this is a problem for the medication that promotes the prevention than treatment strategy for colorectal cancer. That leads to the study of prevention strategy of colorectal cancer in an animal model of colitis induction, which is associated with colorectal cancer event. The objective of the current study was to evaluate the colon cancer-related colitis preventive property of black rice bran, rich in phytochemicals, and beta-glucan from yeast. The rice bran was evaluated the phytochemical content including phenolics, flavonoids, anthocyanins, tocol, and γ-oryzanol. This found that the black rice bran was richest an anthocyanins. Moreover, yeast strain was selected and indentified through 5.8S RNA sequencing. The Saccharomyces cerevisiae from sugarcane was isolated and confirmed. Then isolated S. cerevisiae was studied for beta-glucosidase activity and beta-glucan content. The results found that the optimum condition for beta-glucosidase activity was pH 4, 40 °C, and 0.5%w/v sodium chloride at 24 hours. Besides, the 91.42% w/w of beta-glucan (beta-1, 3 and beta-1, 6) was extracted from yeast cell by the strong base (sodium hydroxide)/week acid (acetic acid) method. Then, the extracted beta-glucan was studied for immunomodulatory effect in BALB/cMlac and found that the intake of 200 mg/kg of body weight for seven days can activate the interleukin-6, interleukin-10, interleukin17, tumor necrosis factor-α, and transforming growth factor-β secretion, significantly highest (p < 0.05) when compared with other doses. Moreover, the fermentation of sterile black rice bran (autoclaved at 90°C for 15 min) with yeast beta-glucosidase producing for 24 hours were standardized with Box-Behnken (BBD) and Response Surface Method (RSM) design. The results found that anthocyanins in black rice bran have changed during fermentation. The anthocyanin (glycoside) was transformed to anthocyanidin (aglycoside) is mechanism related with the biological activity was highest increased at 24 hours but after that anthocyanidin was not stable. Thus, colorectal cancer-related colitis prevention in rats was studied with non-fermented black rice bran extracts, and yeast beta-glucan. The dose of each intervention for 14 days was 500 mg/kg of body weight. The results found that the colitis-induced rat groups have increased in pro-inflammatory cytokines (interleukin-6, interleukin-17, and tumor necrosis factor-α), and a decrease in anti-inflammatory cytokines (interleukin-10 and transforming growth factor-β), and antioxidant enzymes (catalase, superoxide dismutase, and glutathione peroxidase), significantly (p < 0.05). Furthermore, large intestine of colitis induced rat was destroyed especially colon part, whereas black rice bran extracts prevent the pathological signs effectively than beta-glucan (p < 0.05), which was characterized by the decrease in the pro-inflammatory cytokines, and colon tissue destruction, an increase in the anti-inflammatory cytokines secretion and antioxidant enzymes activities. The combination of polar extract of black rice bran and beta-glucan can improve the colon tissue than other interventions. In conclusion, the black rice bran and beta-glucan combinations can prevent and reduce the colon cancerrelated colitis pathological signs. The mechanism behind the recovery and prevention of colitis is possibly due to the anti-oxidative stress in cellular and body system, anti-inflammatory effect of black rice extract, and yeast beta-glucan can improve the immune cells for scavenging the foreign particles, pathogens, and abnormal cells. This study revealed that the black rice bran extracts and yeast beta-glucan are one of the alternative options for colitis prevention and treatment, and colorectal cancer prevention.

Chiang Mai University Library

Address: Chiang Mai

Email: cmulibref@cmu.ac.th

Name: Chutinun Prasitpuriprecha

Role: Chairman

Name: Sasithorn Sirilun

Role: Member

Name: Chaiyaval Chaiyasul

Role: Member

Name: Prasit Suwannakrt

Role: Member

Name: Nantarit Chokethaworn

Role: Member

Created: 2016

Modified: 2020-01-10

Issued: 2020-01-10

วิทยานิพนธ์/Thesis

application/pdf

Th 615.321 N821E

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Pharmacy

Grantor: Chiang Mai University

©copyrights Chiang Mai University

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