Effects of Saraphi (Mammea siamensis) flower extracts on leukemic cell proliferation and expression of wilms’ tumor 1 protein, breakpoint cluster region/abelson, and Fms-like tyrosine kinase 3

ผลของสารสกัดจากดอกสารภี (Mammea siamensis) ต่อการแบ่งตัวของเซลล์มะเร็งเม็ดเลือดขาวและการแสดงออกของโปรตีนวิล์ม ทูเมอร์ 1 เบรคพอยท์คลัสเตอร์รีเจียน/อะเบลซัน และฟิมส์ไลค์ ไทโรซีนไคเนส 3

Name: Rungkarn Sangkaruk

LCSH: Leukemia

LCSH: Proteins

Abstract: Leukemia is a hematopoietic disorder with a frequent incidence and high mortality rate. Previous studies showed that overexpression of Wilms’ tumor 1 (WT1), Breakpoint Cluster Region/Abelson (Bcr/Abl), and Fms-like tyrosine kinase 3 (FLT3) proteins are associated with leukemogenesis. Nowadays, chemotherapy is generally effective treatment and is widely used for leukemia treatment. To avoid the severe side effects of drug chemotherapy, plants having anticancer activity are an alternative medicine for leukemic patients. In this study, Saraphi (Mammea siamensis) flower is a choice of Thai traditional medicine plant. This study aims to investigate the cytotoxic effect of crude ethanolic extract and fractional extracts including hexane, ethyl acetate, and methanol fractions from M. siamensis flowers in human leukemic cell lines; Molt4 (human acute lymphoblastic leukemia), K562 (human chronic myelogenous leukemia), and EoL-1 (human acute myeloblastic leukemia) and to determine their effect on Bcr/Abl protein expression in K562 cells, WT1 protein expression in Molt4, K562, and EoL-1 cells, and FLT3 protein expression in EoL-1 cells. MTT assay was used to determine the cytotoxicity of crude ethanolic extract and three fractional extracts at various concentrations on three leukemic cell lines. The result showed that crude ethanolic extract, hexane, and ethyl acetate fractions were toxic to all cell lines in a dose-dependent manner, while methanol fraction had no cytotoxic effect on three leukemic cell lines. Hexane fraction showed the strongest cytotoxic activity on all cell lines with IC50 values ranging from 2.5 to 2.7 µg/ml in Molt4 cells, 75.1 to 80.1 µg/ml in K562 cells and 3.0 to 4.6 µg/ml in EoL-1 cells. The inhibitory effect of Bcr/Abl, WT1, and FLT3 protein expressions in leukemic cell lines were assessed by Western blot analysis. To determine the effect of effective extracts; crude ethanolic extract, hexane, and ethyl acetate fractions on Bcr/Abl, WT1, and FLT3 protein expressions, all leukemic cell lines were treated with non-cytotoxic concentrations (IC20 values). The result showed that hexane fraction was the best inhibitory effect on Bcr/Abl, WT1, and FLT3 protein expressions in a time- and dose- dependent manner. In summary, hexane fraction, an excellent of Saraphi flower extract, decreased Bcr/Abl, WT1, and FLT3 protein expressions in both time- and dose- dependent manner. The crude, hexane, ethyl acetate and methanolic fractions were analyzed by high performance liquid chromatography (HPLC) as compared to those of ethanolic extract, hexane, ethyl acetate and methanolic fractions to identify the possible compounds in flower extracts. The HPLC fingerprint of a standard mammea E/BB (obtained from M. siamensis seed extract) was used as a standard marker. The hexane fraction contained the highest content of mammea E/BB with the value of 24.38%. Thus, the main active compound in hexane fraction may be the mammea E/BB. These findings suggest that hexane fraction of Saraphi flower extract may be used for alternative treatment in leukemia patients, especially in treating patients with Bcr/Abl, WT1, and FLT3-overexpressing leukemic cells.

Chiang Mai University Library

Address: CHIANG MAI

Email: cmulibref@cmu.ac.th

Name: Trinnakorn Katekunlaphan

Role: Chairman

Name: Songyot Anuchapreeda

Role: Member

Name: Siriporm Okonogi

Role: Member

Name: Sawitree Chiampanichayakul

Role: Member

Created: 2015

Modified: 2020-01-10

Issued: 2020-01-10

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: Th 616.99419 R942E

eng

DegreeName: Master of science

Level: Master's Degree

Descipline: Medical Technology

Grantor: Chiang Mai University

©copyrights Chiang Mai University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	mtech81215rskr_full.pdf	5.28 MB

ใช้เวลา

0.034768 วินาที