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Phan Thi Kim Khanh. Application of plasma technology for elimination of pesticide residues and inactivation of colletotrichum gloeosporioides in mango (CV. Naam Dok Mai). Master's Degree(Food Science and Technology). Chiang Mai University Library. : Chiang Mai University, 2017.
Application of plasma technology for elimination of pesticide residues and inactivation of colletotrichum gloeosporioides in mango (CV. Naam Dok Mai)
Abstract:
Mango (cv. Nam Dok Mai) is considered as a popular and important export fruit of Thailand because of its favorable flavor, attractive color, and high nutritional properties. Nowadays, consumers consciously desire not only fresh and nutritious but also safe fruits with low or free of microorganisms and chemical contamination. Non-thermal plasma (NTP) is one of the emerging technologies that could potentially decontaminate foods and biological materials. Recently, NTP emerges as a new sanitizing method in the agro-industrial applications such as the microbial inactivation and also the elimination of pesticide and toxic chemical residues in fresh food and food processing surface, where the qualitative characteristics of the treated product could probably be modified by the reactive species as well as the residues of oxidation processes. In this study, an applied flow rate of Argon (Ar) gas and time dependent degradation of chlorpyrifos, cypermethrin and carbendazim were observed for NTP treatments produced by gliding arc (GA) discharge with Ar flux of 2, 5 and 8 L/min and treatment duration ranging from 1-15 min. The changes of physicochemical indexes of mango treated with the employed NTP conditions were also evaluated. In addition, the effect of NTP on antifungal activities obtained from different flux of Ar gas (1-5 L/min) and treatment duration (1-7 min) were investigated against mycelium growth, spore number and anthracnose severity on mango of Colletotrichum gloeosporioides. It was found that plasma treatment successfully decreased the concentrations of pesticide by 24.07-74.02 % for chlorpyrifos, 30.42-77.43 % for cypermethrin and 32.31-75.96 % for carbendazim, and physicochemical properties of mango treated NTP were slightly affected. There were significant decreases (p<0.05) in titratable acidity, ascorbic acid and total phenolic content of NTP treated mango, while the total soluble solid and carotenoid values showed a significant increase (p<0.05). However, the moisture content, color and texture parameters of this fruit with NTP treatments were not significantly different (p>0.05). The obtained results also indicated that GA discharge NTP at 5 L/min Ar gas for 7 min provided a high antimicrobial efficiency against the growth of C. gloeosporioides mold on potato dextrose agar and mango fruits under storage conditions at 28-33oC and significantly (p<0.05) reduced the number of this fungus spore in suspension. This study showed that GA discharge NTP is a potential and promising method to ensure microbial and chemical safeties not only for mango, but also for other types of postharvest product.