Antioxidant and hepatoprotective properties of ruellia tuberosa extracts on ethanol-induced toxicity in HepG2 cells

คุณสมบัติต้านออกซิเดชันและการปกป้องตับของสารสกัดต้อยติ่งต่อความเป็นพิษที่เหนี่ยวนำให้เกิดโดยเอทานอลในเซลล์เฮปจี 2

Name: Suphawana Khachitpongpanit

Classification :.DDC: 581.634

LCSH: Plant extracts

LCSH: Antioxidants

LCSH: Ethanol

LCSH: Medicinal plants

Abstract: Ruellia tuberosa is a tropical plant in Acantaceae family that widely distributed in South East Asia and has been used in folk medicine. In the present study, we determined an antioxidant activity and hepatoprotective effects of various parts of Ruellia tuberosa extracts. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical, nitric oxide (NO) radical, hydrogen peroxide (H2O2) scavenging activity, ferric reducing antioxidant power (FRAP) and reducing power assay were used for study antioxidant activities. Additionally, total phenolic, flavonoid, tannin, and alkaloid contents were determined. The results showed the ethanolic leaves extract gave the highest phenolic, flavonoid and tannin contents with the value of 89.66±1.59 mg gallic acid equivalent/100 g extract, 45.35±5.24 mg quercetin equivalent/100 g extract and 68.16±3.15 mg tannic acid equivalent/100 g extract, respectively. The hexane leaves extract showed the highest alkaloid content with the value of 96.32±0.47 mg strychnine equivalent/100 g extract. The ethanolic leaves, root and stem extracts exhibited the highest scavenging potential on DPPH radical and antioxidant model. The highest potency of NO radical scavenging found in chloroform leaves extract, aqueous root and stem extracts. In addition, the ethyl acetate fractions of the leaves, root, and stem were highly scavenge H2O2 molecule. HepG2 cells were used for studying hepatoprotective properties of the ethanolic extracts against ethanol-induced toxicity. The results showed that cytochrome P450 2E1 (CYP2E1), liver enzymes (aspartate aminotransferase, AST and alanine aminotransferase, ALT) activities, malondialdehyde (MDA) and intracellular reactive oxygen species (ROS) were increased whereas reduce glutathione (GSH) was decreased when induced with ethanol. Pretreatment of HepG2 cells with the ethanolic leaves and stem extracts significantly decreased CYP2E1 activity and inhibited the leakage of AST and ALT in HepG2 cells (p < 0.05) but the root extract was increased AST and ALT activities due to the hepatic cells injuries. However, ADH and ALDH activities were not difference in the leaves and root extracts while ALDH activity was induced in the stem extract when compared with the control group or ethanol group. Pretreatment of the cells with the ethanolic extracts significantly decreased ethanol intoxication at p < 0.05. The ethanolic stem extract was the best hepatoprotective agent by inhibit lipid peroxidation, decrease AST and ALT activities, reduce intracellular ROS, and enhance the antioxidant system in the liver. Then, Ruellia tuberosa stem extracts could be uses as a new natural source for using prevent the liver against oxidative stress from drinking ethanol and the mechanism should be studied in the in vivo models.

Chiang Mai University Library

Address: CHIANG MAI

Email: cmulibref@cmu.ac.th

Name: Kongsak Boonyapranai

Role: Chairman

Name: Churdsak Jaikang

Role: Member

Name: Supawatchara Singhatong

Role: Member

Created: 2016

Modified: 2020-01-04

Issued: 2020-01-04

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: Th 581.634 S959A

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Toxicology

Grantor: Chiang Mai University

©copyrights Chiang Mai University

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