Effects of micro nano-bubble for surface sterilization on the survival rate of Rhynchostylis hybrid orchid shoot tips

Name: Dang Cong Du

Address: Lampang, 52000, Thailand

Organization : Department of Plant Science, Faculty of Science and Agricultural Technology, Rajamangala University of Technology Lanna

Email : dangcongdu@gmail.com

Name: Siripun Sarin

Address: Phisanulok, 56000, Thailand

Organization : Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University

Name: Dang Van Dong

Address: Trau Quy-Gia Lam-Ha Noi, Vietnam

Organization : Center for Flowers, Ornamentals Research and Development, Fruit and Vegetable Research Institute

Name: Aphichat Chidburee

Address: Lampang, 52000, Thailand

Organization : Agricultural Technology Research institute, Rajamangala University of Technology Lanna

keyword: Orchid

; In vitro

Abstract: The purpose of this research was to determine the effects of micro nano-bubble for surface sterilization on the survival rate of Rhynchostylis hybrid orchid shoot tips. Explant surface sterilization using micro-nano bubble for 30, 40 and 50 minutes compared with the treatment check, 10% Clorox 10 minutes + treated in 10 minutes micro nano-bubble, and treatment control, 10% Clorox 10 minutes were investigated. All the treatments were cultured on two different media: Vacin and Went (VW) medium and VW medium containing 2 ml/l PTC3 (Plant Tissue Culture Contamination Control). After 7-day culture, the results showed that 100% of microbial contamination occurred on all treatments cultured on VW media, while the explants culture on VW medium contained PTC3 with fungi about 40-60%. The contamination of bacteria in all treatments cultured on VW media + PTC3 was not significant (60-80%). The survival rates of shoot tips of 20 percentage using surface sterilization with micro nano-bubble for 40 and 50 minutes were higher than those of other treatments. The explants treated with micro nano-bubble were green, but the explants of the control were brown. Therefore, this new technique can be applied to disinfect the surface of explants while reducing the expenses as it does not use chemicals.

Rajamangala University of Technology Lanna. Library

Address: CHIANGMAI

Email: library@rmutl.ac.th

Created: 2019

Modified: 2019-10-15

Issued: 2019-10-10

บทความ/Article

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BibliograpyCitation : Book of Proceeding The 10th Rajamangala University of Technology international conference (July,2019), p.37-43

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