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Phenjun Mekvichitsaeng. Production of baculovirus for biopesticide in insect cell culture. III, Effect of temperature oscillation on baculovirus replication . (). King Mongkut's University of Technology Thonburi. : , 1998.
Production of baculovirus for biopesticide in insect cell culture. III, Effect of temperature oscillation on baculovirus replication
Name: Je-nuriah Hayeeawana
Organization :
King Mongkut's University of Technology Thonburi. School of Bioresources and Technology
Name: Kanokwan Poomputsa
Organization :
King Mongkut's University of Technology Thonburi. School of Bioresources and Technology
Name: Phenjun Mekvichitsaeng
Organization :
King Mongkut's University of Technology Thonburi. Pilot Plant Development and Training Institute
keyword:
Baculovirus
Abstract:
In the past years, production of Baculovius as biopesticide using unsect cell culture has gained more attention than the insect larvae system. Major advantages of using insect cell culture are the capabilities to control the quality of the product and to be able to produce in large scale. In this work, we are investigating factors which involved in insect cell growth and viral replication for maximum baculovirus production. Temperature is an important factor affecting insect cell growth and baculovirus replication. The temperature range of 25 to 28 C is favorable to insect cell growth and the optimal is at 27 C. However, Shao-Hua et.al. found that temperature oscillation can enhance cell viability of Sf9 insect cells and AcNPV baculovirus production. Effect of temperature oscillation on Hz (Heliothis zea) insect cell growth and HaNPV baculovirus replication was there fore studied. By changing temperature from normal growth temperature (27 C) to 20, 24 and 30 C at different periods of incubation time (12 and 24 Hours) then transfer to normal growth temperature, cell viability and viral production were recorded. Preliminary result revealed growth enhancing effect when incubated Hz cells (Heliothis zea) at 23 C for 12 hours before changing to normal growth temperature. The results of temperature oscillation on viral replication from the above conditions will be analysed and adapted all optimal conditions for maximum baculovirus productivity
King Mongkut's University of Technology Thonburi
Address:
Bangkok
Email:
info.lib@mail.kmutt.ac.th
Created:
1998
Issued:
2551-03-10
Modified:
2018-11-08
บทความ/Article
BibliograpyCitation :
Compiled Research and Technical Papers/Poster, KMUTT 1998 p. 260-271
CallNumber:
12001351
eng
©copyrights King Mongkut's University of Technology Thonburi
RightsAccess:
ใช้เวลา
0.034005 วินาที
Je-nuriah Hayeeawana
| Title | Contributor | Type |
|---|---|---|
| Production of baculovirus for biopesticide in insect cell culture. III, Effect of temperature oscillation on baculovirus replication
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี Je-nuriah Hayeeawana ;Kanokwan Poomputsa;Phenjun Mekvichitsaeng | บทความ/Article |
Kanokwan Poomputsa
| Title | Contributor | Type |
|---|---|---|
| Production of Baculovirus for biopesticide in insect cell culture I : Optimization for dose of biopesticide
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี Phenjun Mekvichitsaeng;Uthai Ketunuti ;Kanokwan Poomputsa ;Yuwapin Lertwerawat ;Morakot Tanticharoen | บทความ/Article | |
| Production of Baculovirus for biopesticide in insect cell culture
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี Phenjun Mekvichitsaeng ;Kanokwan Poomputsa;Yuwapin Lertwerawat;Uthai Ketunuti;Morakot Tanticharoen | บทความ/Article | |
| Production of baculovirus for biopesticide in insect cell culture. II, Bioassays for research for efficacy comparison
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี Phenjun Mekvichitseang ;Kanokwan Poomputsa ;Uthai Ketunuti ;Morakot Tanticharoen | บทความ/Article | |
| Production of baculovirus for biopesticide in insect cell culture. III, Effect of temperature oscillation on baculovirus replication
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี Je-nuriah Hayeeawana ;Kanokwan Poomputsa;Phenjun Mekvichitsaeng | บทความ/Article | |
| Expression of recombinant dengue viral protein using baculovirus expression insect cell system
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี Imerb Punsod;Kattharity Puewkoaw;Phenjun Mekvichitsaeng;Supapon Cheevadhanarak;Prida Malasit;Kanokwan Poomputsa | บทความ/Article | |
| Pilot scale production of baculovirus using insect cell culture for biopesticides
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี Kanokwan Poomputsa;Kathariya Puewkhow;Phenjun Mekvichitsaeng;Uthai Ketnuti;กนกวรรณ พุ่มพุทรา;แคทริยา ผิวขาว;เพ็ญจันทร์ เมฆวิจิตรแสง;อุทัย เกตุนุติ | บทความ/Article |
Phenjun Mekvichitsaeng
| Title | Contributor | Type |
|---|---|---|
| Production of Baculovirus for biopesticide in insect cell culture I : Optimization for dose of biopesticide
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี Phenjun Mekvichitsaeng;Uthai Ketunuti ;Kanokwan Poomputsa ;Yuwapin Lertwerawat ;Morakot Tanticharoen | บทความ/Article | |
| Production of Baculovirus for biopesticide in insect cell culture
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี Phenjun Mekvichitsaeng ;Kanokwan Poomputsa;Yuwapin Lertwerawat;Uthai Ketunuti;Morakot Tanticharoen | บทความ/Article | |
| Production of baculovirus for biopesticide in insect cell culture. III, Effect of temperature oscillation on baculovirus replication
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี Je-nuriah Hayeeawana ;Kanokwan Poomputsa;Phenjun Mekvichitsaeng | บทความ/Article | |
| Expression of recombinant dengue viral protein using baculovirus expression insect cell system
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี Imerb Punsod;Kattharity Puewkoaw;Phenjun Mekvichitsaeng;Supapon Cheevadhanarak;Prida Malasit;Kanokwan Poomputsa | บทความ/Article | |
| Pilot scale production of baculovirus using insect cell culture for biopesticides
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี Kanokwan Poomputsa;Kathariya Puewkhow;Phenjun Mekvichitsaeng;Uthai Ketnuti;กนกวรรณ พุ่มพุทรา;แคทริยา ผิวขาว;เพ็ญจันทร์ เมฆวิจิตรแสง;อุทัย เกตุนุติ | บทความ/Article |