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Aphichart Karnchanatat. Optimization of preparing protein hydrolysate from Split Gill mushroom (Schizophyllum commune) by alcalase using response surface methodology (RSM). (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2019.
Optimization of preparing protein hydrolysate from Split Gill mushroom (Schizophyllum commune) by alcalase using response surface methodology (RSM)
Name: Aunchalee Wongaem
Organization :
Chulalongkorn University. Faculty of Science
Name: Papassara Sangtanoo
Organization :
Chulalongkorn University. Institute of Biotechnology and Genetic Engineering
Name: Aphichart Karnchanatat
Organization :
Chulalongkorn University. Institute of Biotechnology and Genetic Engineering
keyword:
Optimum condition
Abstract:
The aim of this research was to study the optimum condition for split gill protein hydrolysate production using a microbial protease, Alcalase. The experimentation was conducted using Central Composite Design and Response Surface Methodology (RSM) was used to determine the optimum conditions to obtain the maximum total split gill protein hydrolysate. The three independent variables including hydrolysis temperature hydrolysis time and enzyme to substrate ratio while the pH was fixed at 8. All data were analyzed using Design-Expert Software. The results showed that these three factors significantly affected on the DH and antioxidant activity. However, antioxidant activity was not related in proportion to DH because it depends on the molecular weight, amino acid composition and amino acid sequence of the resulting peptides. The optimum conditions, under which the highest ABTS radical scavenging activity and DH value of 50.882 percent were obtained, were under 55 Celsius degrees, 157 minutes hydrolysis time and 2 percent v per w of enzyme per substrate ratio. The coefficient of determination (R2) was 0.972. Due to the high antioxidant potential, hydrolysate was fractionated by molecular weight cut-off membranes. The below 0.65 kDa fraction showed the highest scavenging ability with IC50 value of 0.026 ug per mL. These results suggest that split gill mushroom protein hydrolysate is good for use as a source of bioactive peptide that has potential as a natural antioxidant in food production and ingredient in cosmetic products
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2019
Modified:
2019-09-30
Issued:
2019-09-30
บทความ/Article
application/pdf
BibliograpyCitation :
In Chemical Society of Thailand under the Patronage of Professor Dr. HRH Princess Chulabhorn. Pure and Applied Chemistry International Conference 2019 (PACCON 2019) (FA87-93). Bangkok : Mahidol University
eng
©copyrights King Mongkut's University of Technology North Bangkok
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0.033777 วินาที
Aunchalee Wongaem
| Title | Contributor | Type |
|---|---|---|
| Optimization of preparing protein hydrolysate from Split Gill mushroom (Schizophyllum commune) by alcalase using response surface methodology (RSM)
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Aunchalee Wongaem;Papassara Sangtanoo;Aphichart Karnchanatat | บทความ/Article | |
| Optimization of protein hydrolysate preparation from split gill mushroom Schizophyllum commune
จุฬาลงกรณ์มหาวิทยาลัย Aunchalee Wongaem | Aphichart Karnchanatat | วิทยานิพนธ์/Thesis |
Papassara Sangtanoo
| Title | Contributor | Type |
|---|---|---|
| Free radical scavenging properties and DNA damage protecting of peptide hydrolysate derived from edible mushroom Astraeus hygrometricus
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Natpatsorn Tunyatarinun;Onrapak Reamtong;Papassara Sangtanoo;Tanatorn Saisavoey;Aphichart Karnchanatat | บทความ/Article | |
| Optimization of preparing protein hydrolysate from Split Gill mushroom (Schizophyllum commune) by alcalase using response surface methodology (RSM)
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Aunchalee Wongaem;Papassara Sangtanoo;Aphichart Karnchanatat | บทความ/Article | |
| Optimization of shiitake mushroom (lentinula edodes) hydrolysate with angiotensin I converting enzyme inhibitory activity
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Supawee Paisansak;Papassara Sangtanoo;Aphichart Karnchanatat | บทความ/Article |
Aphichart Karnchanatat