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Thanyaporn Wongnate. Overexpression of pyranose dehydrogenase in E.coli platform. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2019.
Overexpression of pyranose dehydrogenase in E.coli platform
Name: Pangrum Punthong
Organization :
Vidyasirimedhi Institute of Science and Technology. School of Biomolecular Science and Engineering
Name: Litavadee Chuaboon
Organization :
Mahidol University. Faculty of Science
Name: Jeerus Sucharitakul
Organization :
Chulalongkorn University. Faculty of Dentistry
Name: Pimchai Chaiyen
Organization :
Vidyasirimedhi Institute of Science and Technology. School of Biomolecular Science and Engineering
Name: Thanyaporn Wongnate
Organization :
Vidyasirimedhi Institute of Science and Technology. School of Biomolecular Science and Engineering
Email :
thanyaporn.w@vistec.ac.th
keyword:
Pyranose dehydrogenase
LCSH:
E. coli infections
LCSH:
Food contamination
Abstract:
Pyranose dehydrogenase (PDH) catalyzes the oxidation of aldopyranoses by using ferricenium ion or 1,4-benzoquinone as electron acceptors to yield the corresponding ketoaldoses. This enzyme contains flavin adenine dinucleotide as a cofactor and has been proposed to be the key biocatalysts in biotransformation of carbohydrates. PDH can potentially convert sugars to various keto-sugar intermediates, which can be used in the synthesis of rare sugars, fine chemicals and drugs. Based on our study, expressing recombinant PDH in E. coli has been difficult because of low solubility and low yield issues. We found that E. coli BL21(DE3) strain could be used to simultaneously express PDH and groEL groES chaperone from two plasmids, resulting in the improvement of the recombinant PDH solubility. Our results could facilitate the production of soluble and active recombinant PDH for further functional and biocatalysis efficiency studies.
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2019
Modified:
2019-08-29
Issued:
2019-08-29
บทความ/Article
application/pdf
BibliograpyCitation :
In Chemical Society of Thailand under the Patronage of Professor Dr. HRH Princess Chulabhorn. Pure and Applied Chemistry International Conference 2019 (PACCON 2019) (CB7-11). Bangkok : Mahidol University
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.023437 วินาที
Pangrum Punthong
| Title | Contributor | Type |
|---|---|---|
| Overexpression of pyranose dehydrogenase in E.coli platform
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Pangrum Punthong;Litavadee Chuaboon;Jeerus Sucharitakul;Pimchai Chaiyen;Thanyaporn Wongnate | บทความ/Article |
Litavadee Chuaboon
| Title | Contributor | Type |
|---|---|---|
| Overexpression of pyranose dehydrogenase in E.coli platform
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Pangrum Punthong;Litavadee Chuaboon;Jeerus Sucharitakul;Pimchai Chaiyen;Thanyaporn Wongnate | บทความ/Article |
Jeerus Sucharitakul
| Title | Contributor | Type |
|---|---|---|
| Kinetic and mechanistic studies on p-Hydroxyphenylacetate hydroxylase from acinetobacter baumannii
มหาวิทยาลัยมหิดล Jeerus Sucharitakul | Pimchai Chaiyen | วิทยานิพนธ์/Thesis |
| Overexpression of pyranose dehydrogenase in E.coli platform
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Pangrum Punthong;Litavadee Chuaboon;Jeerus Sucharitakul;Pimchai Chaiyen;Thanyaporn Wongnate | บทความ/Article | |
| Expression and characterization of novel aldehyde deformylating oxygenase from Pseudomonas plecoglossicida
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Supacha Buttranon;Juthamas Jaroensuk;Jeerus Sucharitakul;Pimchai Chaiyen;Thanyaporn Wongnate | บทความ/Article |
Pimchai Chaiyen
| Title | Contributor | Type |
|---|---|---|
| Theoretical study of oxidative half-reaction mechanism of p-Hydroxyphenylacetate 3-Hydroxylase (HPAH)
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Surawit Visitsatthawong;Pimchai Chaiyen;Panida Surawatanawong | บทความ/Article | |
| Overexpression of pyranose dehydrogenase in E.coli platform
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Pangrum Punthong;Litavadee Chuaboon;Jeerus Sucharitakul;Pimchai Chaiyen;Thanyaporn Wongnate | บทความ/Article | |
| Expression and characterization of novel aldehyde deformylating oxygenase from Pseudomonas plecoglossicida
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Supacha Buttranon;Juthamas Jaroensuk;Jeerus Sucharitakul;Pimchai Chaiyen;Thanyaporn Wongnate | บทความ/Article | |
| Characterization of 2-oxo-hept-3-ene-1,7-dioic acid hydratase from Acinetobacter baumannii
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Wachirawit Chinantuya;Pirom Chenprakon;Ruchanok Tinikul;Pimchai Chaiyen | บทความ/Article |
Thanyaporn Wongnate
| Title | Contributor | Type |
|---|---|---|
| Overexpression of pyranose dehydrogenase in E.coli platform
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Pangrum Punthong;Litavadee Chuaboon;Jeerus Sucharitakul;Pimchai Chaiyen;Thanyaporn Wongnate | บทความ/Article | |
| Expression and characterization of novel aldehyde deformylating oxygenase from Pseudomonas plecoglossicida
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Supacha Buttranon;Juthamas Jaroensuk;Jeerus Sucharitakul;Pimchai Chaiyen;Thanyaporn Wongnate | บทความ/Article |