The optimum conditions for extraction of fatty acids from Mon Thong durian seed kernel and analysis by gas chromatography-flame ionization detector

Name: Sirirat Chanvaivit

Organization : Burapha University. Faculty of Science

Email : sirirat@buu.ac.th

Name: Sasimaporn Hangpai

Organization : Burapha University. Faculty of Science

keyword: Mon Thong durian seed kernel

LCSH: Fatty acids -- Analysis

; Flame ionization detector

LCSH: Gas chromatography

Abstract: The extraction conditions for fatty acids from Mon Thong durian seed kernel from Rayong province were studied. Two grams of dry ground seeds was extracted with 50 mL of dichloromethane: methanol in different ratio. The optimum conditions were found as follows: without soak sample before extraction was better than soak sample, sonication sample with dichloromethane: methanol in the ratio of 2:1, sonication time 20 minutes and methylation time via esterification (at 80 oC) for 6 hours. The methylated samples were analyzed by gas chromatography- flame ionization detector with DB-225 column (20 m length, 0.01 mm. ID., and 0.10 um film thickness). GC-FID condition was performed as follows: injector and detector temperature at 240 oC, split ratio 40:1. The oven temperature was 150 oC held for 0.5 min., then increase with the rate of 100 oC/min. to 200 oC followed by the rate of 60 oC/min to 220C and held for 14 min. The last fatty acid came out at 15.33 min. Seven fatty acids were found as follows: palmitic acid (C16:0) 37.95 + 0.08%, palmitoleic acid (C16:1n7) 2.48 + 0.09%, stearic acid (C18:0) 4.16 + 0.04%, vaccenic acid (C18:1n7) 1.43 + 0.02%, oleic acid (C18:1n9) 7.19 + 0.13%, linoleic acid (C18:2n6) 14.73 + 0.07% and a-linolenic acid (C18:3n3) 18.87 + 0.32% respectively. The result showed that Mon Thong durian seed can be used as an alternative way as fatty acid source from waste seed.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2017

Modified: 2562-08-05

Issued: 2019-08-05

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BibliograpyCitation : In Chemical Society of Thailand under the Patronage of Her Royal Hghness Princess Chulabhorn Mahidol and King Mongkut's University of Technology North Bangkok Faculty of Applied Science. Pure and Applied Chemistry International Conference (PACCON 2017) (pp.308-313). Bangkok : Chemical Society of Thailand under the Patronage of Her Royal Hghness Princess Chulabhorn Mahidol

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