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Jesdawan Wichitwechkarn. The detection of organophosphate insecticide using immobilized whole cells from the clone carrying mpdB gene expressing methyl parathion hydrolase. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2018.
The detection of organophosphate insecticide using immobilized whole cells from the clone carrying mpdB gene expressing methyl parathion hydrolase
Name: Chadaporn Pootawee
Organization :
Silpakorn University. Faculty of Engineering and Industrial Technology
Name: Witsanu Senbua
Organization :
Silpakorn University. Faculty of Engineering and Industrial Technology
Name: Chaiyong Taechapairoj
Organization :
Silpakorn University. Faculty of Engineering and Industrial Technology
Name: Jesdawan Wichitwechkarn
Organization :
Silpakorn University. Faculty of Engineering and Industrial Technology
Email :
wjesdawan@gmail.com
keyword:
Organophosphate
LCSH:
Insecticide research
Abstract:
Because of high contamination in agricultural products and the environment of
organophosphate (OP) insecticide, we are interested in studying a simple and convenient
method for OP detection, using methyl parathion (MP) as a representative insecticide. Methyl
parathion degrading, mpdB, gene from MP-degrading Burkholderia cepacia was previously
cloned and expressed in Escherichia coli as a fusion protein of methyl parathion hydrolase
(MPH) and glutathione-S-transferase (GST) [MPH-GST]. The MPH-GST was capable of
degrading MP and other phenyl-substituted OP to a yellow product p-nitrophenol (PNP).
Whole cells from this clone were used to detect MP by spectrophotometrically measuring
PNP at OD410. The cells were optimally immobilized onto the polystyrene-microtiter plate,
using physical adsorption followed by cross-linking with glutaraldehyde. Scanning electron
microscope analysis revealed numerous cells of rod shape binding to the plate surface. The
temperature at which the enzyme activity was most stable was 25°C. This bio-component
system could be used to detect MP repetitively up to 46 rounds. The MP detection limit of
this system was 2 μg/mL, and the linear range was 2-200 μg/mL. This whole cell
immobilization system has a great potential to be utilized for the detection of OP insecticides
with ease, convenience and low cost.
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2018
Modified:
2562-07-04
Issued:
2019-07-04
บทความ/Article
application/pdf
BibliograpyCitation :
In Prince of Songkla University Faculty of Science. Pure and Applied Chemistry International Conference (PACCON 2018) (p.FA83-FA87). Songkla : Prince of Songkla University
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.030505 วินาที
Chadaporn Pootawee
| Title | Contributor | Type |
|---|---|---|
| The detection of organophosphate insecticide using immobilized whole cells from the clone carrying mpdB gene expressing methyl parathion hydrolase
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Chadaporn Pootawee;Witsanu Senbua;Chaiyong Taechapairoj;Jesdawan Wichitwechkarn | บทความ/Article |
Witsanu Senbua
| Title | Contributor | Type |
|---|---|---|
| The detection of organophosphate insecticide using immobilized whole cells from the clone carrying mpdB gene expressing methyl parathion hydrolase
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Chadaporn Pootawee;Witsanu Senbua;Chaiyong Taechapairoj;Jesdawan Wichitwechkarn | บทความ/Article |
Chaiyong Taechapairoj
| Title | Contributor | Type |
|---|---|---|
| การอบแห้งข้าวเปลือกโดยเทคนิคฟลูอิไดซ์เบด้วยไอน้ำร้อนยวดยิ่ง
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี ชัยยงค์ เตชะไพโรจน์;Chaiyong Taechapairoj | Somchart Soponronnarit | วิทยานิพนธ์/Thesis |
| The detection of organophosphate insecticide using immobilized whole cells from the clone carrying mpdB gene expressing methyl parathion hydrolase
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Chadaporn Pootawee;Witsanu Senbua;Chaiyong Taechapairoj;Jesdawan Wichitwechkarn | บทความ/Article |
Jesdawan Wichitwechkarn
| Title | Contributor | Type |
|---|---|---|
| The detection of organophosphate insecticide using immobilized whole cells from the clone carrying mpdB gene expressing methyl parathion hydrolase
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Chadaporn Pootawee;Witsanu Senbua;Chaiyong Taechapairoj;Jesdawan Wichitwechkarn | บทความ/Article |