พิพัฒน์ เกษมชัยชุติพันธ์. Investigation on Microbial Augmentation in Anaerobic Dechlorination of Hexachlorobenzene. Master's Degree(Environmental Engineering). King Mongkut's University of Technology Thonburi. KMUTT Library. : King Mongkut's University of Technology Thonburi, 2011.
Investigation on Microbial Augmentation in Anaerobic Dechlorination of Hexachlorobenzene
Abstract:
This research aimed to investigate the capability of indigenous anaerobic consortia in
HCB dechlorination by using the sediments from Erh-Jen River, in Tainan County of
southern Taiwan, and four stream sediments in Samut Prakarn Province of Thailand.
Effects of bioaugmentation and biostimulation were also studied. The experiments were
conducted in serum bottles under anaerobic condition at room temperature and in the
dark. The effect of mixing consortia between non-active HCB-dechlorinating consortia
(sediments from Taiwan) and active HCB-dechlorinating consortia (sediments from
Thailand) was studied. Bioaugmentation experiments were carried out in two scenarios:
a) stored sediments from Thailand at 6 C for 10 months mixed with sediments from
Taiwan; and b) fresh sediments from Thailand mixed with sediments from Taiwan. For
biostimulation study, the sterilized sediments from Thailand were mixed with sediments
from Taiwan. The results showed that extensively stored sediments could not enhance
the microbial activity in RCB dechlorination even with yeast extract supplement.
Supplement with essential substrates/nutrients through sterilized active sediments could
effectively promote the HCB dechlorination rate in the non-active sediments. Addition
of actively HCB-dechlorinatlng consortia to non-active consortia could not enhance the
performance ofHCB dechlorination, indicating that sufficient substrates/nutrients was a
key factor affecting the HCB dechlorination rate.