การออกแบบและสร้างกลูโคสเซนเซอร์โดยเทคนิคการพิมพ์สกรีน

Design and Construction of Glucose Sensors by Screen Printing Technique

Name: นิยม ทองนิมิตร

Name: Niyom Thongnimit

Organization : มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

keyword: Glucose

; Glucose Sensor

; Mediator

; 1,l'-dimethylferrocene

; Seen Printing Technique

Abstract: The objective of this thesis is to study how to use the screen printing technique for fabrication of glucose screen-printed sensor. Patterns of two electrochemical electrodes and dielectric insulation were designed. A heat-stabilised polyvinylchloride (PVC) substrate sheet was firstly screened with carbon paste ink as a conducting track. The second layer was composed of graphite paste as a worlting electrode, commercial silverlsilver chloride paste as a reference electrode, and commercial dielectric paste as an insulation layer was screened, respectively. The working electrode was obtained from a mixture of graphite powder and printing solution (2 percent wlv hydroxyethyl cellulose and 6 percent wlv ethylene glycol). In this study, the screen-printed AgIAgC1 reference electrode showed electrocheinical behaviour which was similar to a coimnercial AgfAgCl reference electrode. Also, an electrocheinical behaviour of 1 ,l '-dimct11ylfcrr;ccne (dinfc) dricd ovcr thc working electrode was observed in 0.1 M pllosphate buffer cont~ining0.1 M KCl. After applying 1,l '-dimethylferrocene and left for drying, a 4 yl of a mixture solution containing 10 unitslml of glucose oxidase and 2 percent glutaraldehyde. was dropped over the working electrode. The response of the electrode system was assessed via areaction undergoing between the enzyme, glucose and the redox mediator. The screen-printed AgIAgC1 reference electrode has the electrochemicnl behnviour siinilar to AgIAgC1 commercial reference electrode. T11c glucose measureinent was based on the ainperometric technique. By applying +300 inV vs AgIAgC1, the linear response to glucose solution was obtained across the concentration range 3-6 inM or 54-108 mgldl with a sensitivity of 0.0583 pA/(mg/dl). Each sample analysis took about 60 seconds and the average results of the tests has 95 percent in confidence interval. The glucose sensor showed good reproducibility, as its standard deviation of glucose 0.5 mM (9 mgldl), 4 mM (72 ingtdl) and 7 inM (126 mg/dl) were 0.033, 0.045 and 0.04 1, respectively (n = 10).

มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

Address: กรุงเทพมหานคร

Email: info.lib@mail.kmutt.ac.th

Name: วีระศักดิ์ สุระเรืองชัย

Role: อาจารย์ที่ปรึกษา

Created: 2543

Issued: 2005-11-17

Modified: 2556-04-13

วิทยานิพนธ์/Thesis

ISBN: 9746283251

CallNumber: PRE730

tha

DegreeName: วิศวกรรมศาสตรมหาบัณฑิต

Level: ปริญญาโท

Descipline: วิศวกรรมอุตสาหการ

Grantor: มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

©copyrights มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

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