Studies on fungal lipases for interesterification of palm oil

การศึกษาเอนไซม์ไลเปสจากเชื้อราเพื่อใช้เร่งอินเตอร์เอสเตอริฟิเคชั่นในน้ำมันปาล์ม

Name: Napawan Promkhatkaew

LCSH: Enzymes, Immobilized

LCSH: Fatty acids, Nonesterified

LCSH: Fungi

LCSH: Lipase

LCSH: Palm-oil

Abstract: Attempts were made to find out efficient lipase producers from five strains of fungi, namely Aspergillus niger 3240, A. niger MUCA, A. niger 3092, Mucor TISTR and Rhizopus delemar. Of these, R. delemar grown in submerged culture produced lipase of high activity (~800 unit/ml) and A. niger 3240 grown on wheat bran-based semi-solid culture also produced subtantial amount of lipase (1400 unit/g medium). Crude lipase in the culture extract of A. niger 3240 had optimal pH at 5, optimal temperature at 30 C and heat stability up to 60 C. The enzyme was purified by ammonium sufate preciptation followed by DEAE-cellulose and Sephadex G-100 column chromatography. The yield obtained was 43% with 16 fold purification. The partially purified lipase was immobilized on celite for being used in intereste-rification reaction of palm oil with [1-14C] -stearic acid. The celite-adsorbed lipase had pH stability in the range of 3-9 and catalyzed incorporation of [1-14C] -stearic acid into triglyceride of palm oil at optimum temperature 45 C, optimum pH 3-5 and was favorable to catalyze interesterification of triglycerides having long-chain fatty acid in water-saturated n-hexane. The immobilized lipase was shown to be able to catalyze interesterification of palm oil with stearic, linoleic and arachidonic acids. The interesterified product analyzed by gas liquid chromatography showed the replacement of palmityl and oleoyl moieties in trigly-cerides of palm poil with the added fatty acids. Further work is required to improve efficiency of lipase-catalyzed interesterification of paim oil.

Mahidol University. Mahidol University Library and Knowledge Center

Address: NAKHONPATHOM

Email: liwww@mahidol.ac.th

Name: Prayad Komaratat

Role: Thesis Advisors

Name: Prapon Wilairat

Role: Thesis Advisors

Name: Amaret Bhumiratana

Role: Thesis Advisors

Name: Pichit Tosukhowong

Role: Thesis Advisors

Created: 1990

Modified: 2018-10-10

Issued: 2018-10-10

วิทยานิพนธ์/Thesis

application/pdf

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biochemistry

Grantor: Mahidol University

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