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Suwanit Suwannavong. Study on toxicogenesis of T-2 toxin. Master's Degree(Toxicology). Mahidol University. Mahidol University Library and Knowledge Center. : Mahidol University, 1987.
Study on toxicogenesis of T-2 toxin
การศึกษากลไกการเกิดพิษของสารพิษ T-2
Name: Suwanit Suwannavong
MeSH:
Calcium
MeSH:
Cytotoxins
MeSH:
Dexamethasone
MeSH:
Trichothecenes
-- toxicity
Abstract:
T-2 toxin cytotoxicity was studied both in vitro under different calcium concentration and in vivo. During in vitro study AS-30D cells were incubated in the presence of T-2 toxin (0.5 ug/ml) in a different calcium concentration (0, 1.5 and 3.0 mM). The cytotxicity of T-2 toxin as well as the influence of isoptin was evidenced by vital dye uptake study, leakage of enzyme and the changes in cellular level of adenine uncleotides. The results revealed an increase of vital dye stained cells, the leakage of LDH into incubation medium and the decrease in cellular level of ATP concomitantly with the increased ADP and AMP levels. This evidence also supported by the decreased energy charge in T-2 treated AS30D cell. These cytotoxicities in duced by T-2 toxin could effectively be prevented by isoptin at all calcium concentrations studied. In the animal, T-2 toxin significantly induced an increase in calcium uptake to the skin exposed to T-2 toxin topically. This effect could effectively be reduced by either isoptin or dexamethasone. The result obtained indicated at least 2 concerted mechanisms involving in the toxicity of T-2 toxin. The calcium dependent cytotoxicity that could effectively be prevented by isoptin and the calcium independent cytotoxic effect. The precise mechanism remains to be further elucidated.
Mahidol University. Mahidol University Library and Knowledge Center
Address:
NAKHONPATHOM
Email:
liwww@mahidol.ac.th
Role:
Thesis Advisors
Name: Somphong Sahaphong
Role:
Thesis Advisors
Created:
1987
Modified:
2018-10-03
Issued:
2018-10-03
วิทยานิพนธ์/Thesis
application/pdf
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Toxicology
Grantor: Mahidol University
ใช้เวลา
-0.586644 วินาที
Suwanit Suwannavong
| Title | Contributor | Type |
|---|---|---|
| Study on toxicogenesis of T-2 toxin
มหาวิทยาลัยมหิดล Suwanit Suwannavong | Tongtavuch Anukarahanonta Somphong Sahaphong | วิทยานิพนธ์/Thesis |
Tongtavuch Anukarahanonta
Somphong Sahaphong