Screening for Flocculant-Producing Microorganisms and Their Application for Wastewater Treatment

Name: สายทอง แก้วฉาย

Organization : มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

keyword: bioflocculant

; screening

; metal uptake

; nickel

; cadmium

; POME

Abstract: Screening for polymer-producing microorganisms possessing flocculating activity was carried out. Among fifty-four isolates, three strains WD 90, SM 29, and SM 38 were selected based on their high flocculating activities (24.81, 14.63, and 10.84 respectively) and flocculation rates (94.29, 90.69, and 87.84% respectively). These three strains were identified as Bacillus subtilis WD 90, Bacillus subtilis SM 29, and Enterobacter agglomerans SM 38. Treatment of palm oil mill effluent (POME) by the three selected isolates revealed that no flocculation of total solids and suspended solids was observed. However, all strains were able to decolorize the POME from dark-brown to light-yellow. Bioflocculant produced by the three selected isolates could not separate the suspended solids and oil from the POME. Heavy metal uptake by the cells and bioflocculant of the three selected isolates were studied. The optimum pH for nickel and cadmium uptake by the dry cells of E. agglomerans SM 38 were found to be 7.0 and 8.0, respectively, and pH 8.0 for both metals by the other two strains. The uptakes of nickel and cadmium by the dry cells and wet cells of E. agglomerans SM 38 slightly increased with increasing initial concentration to 60 and 30 ppm, respectively. The bioflocculant of B. subtilis WD 90, and B. subtilis SM 29 showed the highest uptake of nickel and cadmium of 90.73 and 87.07%, respectively, while the cadmium removal was 90.95 and 91.44%, respectively. The optimum pH for nickel and cadmium uptake by the bioflocculant of E. agglonzerans SM 38 was 7.0 and their removal was 92.80 and 84.21%, respectively. The effect of nickel concentrations on the bioflocculant of E. agglomerans SM 38 was highest at 10 ppm (92.51%), and rather stable up to 60 ppm. The optimum cadmium concentration for uptake by the bioflocculant of B. subtilis SM 29, at pH 8.0 was 60 ppm with the removal of 85.72%. The metal uptake by the bioflocculant of the three selected isolates was therefore higher than those by the cells.

มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

Address: กรุงเทพมหานคร (Bangkok)

Email: info.lib@mail.kmutt.ac.th

Name: Assoc.Prof.Dr. Poonsuk Prasertsan

Role: Supervisor

Created: 2543

Issued: 2005-11-08

Modified: 2552-09-01

วิทยานิพนธ์/Thesis

ISBN: 9746286714

CallNumber: JGSEE13

tha

DegreeName: Master of Science

Level: Master's Degree

Descipline: The Joint Graduate School of Energy and Environment

Grantor: King Mongkut's University of Technology Thonburi

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