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Application of Thermodynamic Parameters in GasChromatographic Retention and GCxGC

Name: กรณ์กนก อายุสุข

Organization : มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

Name: Kornkanok Aryusuk

keyword: Gas Chromatographic

Abstract: Eq. (1) and its rearranged form are powerful tool for identification of fatty acid methylesters (FAMEs) and general organic compounds both in isothermal and temperature programmedgas chromatography (GC & TPGC).where k is retention factor, z is carbon number or equivalent chain length (ECL), T isabsolute temperature (K) and a, b, c and d are thermodynamically related columnconstants.The correctness in identification by using Eq. (1) depends very much on the fourthermodynamically related column constants (a, b, c and d). Thus, in this study, theeffects of column sizes and inert carrier gas types on these constants are experimentalverified.The working equations for forecasting retention times of FAMEs and n-alkanes, elutedfrom BPI columns of different inside diameters, could simply be obtained by adding thevalue to the constant a of Eq. (1) whilst the constants b, c and d are unchanged.'Ihc column constants of Eq. ( I ) and the retention factors of FAMEs are affected by thenature of the inert carrier gases. This effect is morc pronounced when the polarstationary liquid phasc (BPX70 column) is used and the k values increase in the order ofAr. The magnitudc of this effect depends on the column polarity and thcdeviation from the ideal state of the gases. Thus, for more accurate identification, it isrecommended that individual set of the numerical constants should be used.In addition, Eq. ( I ) can be used to determine the equivalent temperaturc of FAMEsfrom Chinese mustard seed oil (Brassica juncea) analyzed on BPX70 in one-, two-,three- and four-step TPGC. is used as the reference temperature for calculation ofKovats retention index or in TPGC and may facilitate identification in TPGC.In addition to conventional GC, the high-resolution GC technique namedcomprehensive two-dimensional gas chromatography (GCxGC) is studied and added aspart of in this thesis. GCxGC is shown to provide information on dynamic molecularinterconversion of oximes with the interconversion process occurring on both primaryand secondary columns. The experiment requires suitable adjustment of bothexperimental conditions and relative dimensions of each column. In this study, a longercolumn than that normally used in GCxGC is employed to provide sufficient retentionduration on the second column. The longer retention time permits the typical plateau shapefor the interconversion process to be observed. The extent of interconversiondepends on prevailing temperature, retention time, and the stationary phase type. It wasobserved that the interconversion rate of acctaldoximc and butyraldoximc proceeded inthe following order: BP20 > BP21 > BP10 > BP X5 CP-Chirasil-Dex CB(cnanlioselective column).

มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

Address: กรุงเทพมหานคร (Bangkok)

Email: info.lib@mail.kmutt.ac.th

Name: Assoc.Prof. Kanit Krisnangkura

Role: Supervisor

Created: 2546

Issued: 2005-10-12

Modified: 2006-05-09

วิทยานิพนธ์/Thesis

ISBN: 9741835027

CallNumber: BCT83

tha

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Biochemical Technology

Grantor: King Mongkut's University of Technology Thonburi

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