การใช้ไบโอลิชชิ่งในการสกัดแร่ทองแดงซัลไฟด์เกรดต่ำ

Bioleaching of Low Grade Copper Sulphide ores

Name: ทองบรอนซ์ หอมทอง

Organization : มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

keyword: Bioleaching

; Chalcocite

; Copper

; Thiobacillws fen-ooxidans

; ไบโอลิชชิ่ง

; คาลโคไซด์

; ทองแดง

Abstract: The objective of this research was to investigate the optimum conditions forextraction of low grade copper sulfide ores from Phuthep Company Ltd., Amphae Muang,Leay province, Thailand by Thiobacillws fen-ooxidans DSM583 using shake flask and columnexperiments. The adsorption of microorganisms on mineral surfaces was also investigated. Thiscopper sulfide ores contained 0.07-O-17% copper, l-5% iron, 13-20% sulfur, and pH of 4-8.A preliminary study was conducted in order to determine the feasibility of usingT. ferrooxidans to recover copper from chalcocite ores. Approximately 50% of copper in theminerals dissolved in the water. The remaining insoluble copper was extracted using 0.245g sulphuric acid/g of ore.The copper sulfide ores having particle sizes of less than 38 pm were used inthe shake flask experiments at the 10% pulp density and 10% inoculum in 9K medium.Copper was extracted completely within 14 days when 7’. fenooxidans were added. Without7’. ferrooxidans, 73% of total copper in chalcocite was extracted in 14 days.Particle size of less than 150 p, 10% pulp density, 10% inoculum and initial pHvalue of 2.8 were found to be the optimum conditions in shake flask experiments. Increasingof temperature from 30 ‘C to 37 ‘C did not inhibit the growth of 7’. fen-ooxidans and theaddition of CO, did not enhance the bioleaching rate. Leaching rate was reduced when initialconcentrations of copper in the experiments were higher than 17Og/L. Distilled water with :ferrous ion at pH 2.8 was found to be sufficient for the growth of microorganisms and theleaching of copper from chalcocite. Leaching rate was found to be increased when 4 gof Ag+/kg of ores was used even though it is toxic to T. ferrooxidans.The adsorption rate of 7’. ferrooxidans onto the ore particles was found to be fairlyrapid compared with the leaching rate. The adsorption process was completed within 30minutes. The maximum concentration of bacteria adsorbed on the mineral surface, Xm, was ,1.2784X104 ug protein/g mineral. The adsorption equilibrium constant, Km, was 0.01978 ml/ugprotein.Non-steriled ore with the particle size of 500-l ,180 pm was used in the columnexperiments. Effects of leaching solution used in the experiments were compared. Distilledwater at pH 2.8, distilled water with ferrous ion added at pH 2.8 and 9K medium at pH 2.8were used as leaching solution in the experiments. Distilled water at pH 2.8 was found to givethe highest leaching rate. Jarosite was found in the experiments using leaching solutioncontaining ferrous ion which resulted in lowering the leaching rate. Experiments using distilledwater at pH 2.8 with and without T. fen-ooxidans were compared. The result showed thatwith T. ferrooxidans, the leaching rate was faster in the beginning but total amount of copperleached was the same of that without T. fen-ooxidans added. The leaching rate in the columnexperiments were found to be decreased after 120 days. This could be the result of theaccumulation of sulfur on the surface of ores since yellow sediment was found in thecolumns. From plate count, it was observed that most of microorganisms were found on thesurface of the particles. This showed that leaching mechanisms may involved direct process.

มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

Address: กรุงเทพมหานคร (Bangkok)

Email: info.lib@mail.kmutt.ac.th

Name: ผศ.ดร.ไพทิพย์ ธีรเวชญ

Role: อาจารย์ที่ปรึกษา

Name: รศ.ดร.มรกต ตันติเจริญ

Role: อาจารย์ที่ปรึกษา

Created: 2540

Issued: 2005-04-09

Modified: 2006-05-30

วิทยานิพนธ์/Thesis

ISBN: 9746240889

CallNumber: BIT208

tha

DegreeName: วิทยาศาสตรมหาบัณฑิต

Level: ปริญญาโท

Descipline: เทคโนโลยีชีวภาพ

Grantor: มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

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