ผลของอุณหภูมิต่อองค์ประกอบของกรดไขมันบน Plasma และ Thylakoid Membrane ในสาหร่ายเกลียวทองสายพันธุ์ Z19/2

Effect of Temperature on Fatty Acid Composition of Plasma and Thylakoid Membranes in Spirulina Platensis Strain Z19/2

Name: กัลยาณี ไพฑูรย์รังสฤษดิ์

Organization : มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

keyword: Spirulina platensis

; Fatty acid desaturation

; Temperature

; plasma membrane

; การเติมพันธะคู่ของกรดไขมัน

Abstract: In this work, the effect of temperature on changes of fatty acid composition inlipid membranes was studied in Spirulina platensis Z19/2.When the cells of Spirulina platensis Z19/2 grown at 35’C (optimal growthtemperature) and 25’C, the patterns of growth were not distinct. However, the 35’C-growncells reached exponential phase faster than the 25’C-grown cells. In the 35’C-grown cells,the proportion (% of fatty acid / total fatty acid) of oleic acid (C18:l (9)) and linoleic acid(C 18:2 (9,12)) increased, whereas the proportion of y-linolenic acid Cl 8:3 (6,9,12) decreasedwhen the cells were at stationary phase. In contrast, there was no significant change in fattyacid composition in the 25’C-grown cells. According to these phenomena, the fatty acidcomposition of plasma and thylakoid membranes isolated from 35’C- and 25’C-grown cellswere analyzed. It was found that the proportion of Cl&3 (6,9,12) decreased in plasma andthylakoid membranes of 35’C-grown cells. Whereas, the proportion of C18:3 (6,9,12) inplasma and thylakoid membranes of 25’C-grown cells was almost the same at all growthphase. It suggests that C18:3 (6,9,12) was essential for growth of S. platensis at 25’CThe response of cells of S. platensis Z19/2 to change of temperature from 35 to25’C was also studied, When cells were shifted from 35 to 25’C, the extent of fatty aciddesaturation of plasma membranes occurred within 12 hours after the shift. The increase infatty acid desaturation of thylakoid membranes could be detected after 24 hours of the shift,however, the degree of fatty acid desaturation was lower than that of plasma membranes.By analysis of fatty acid composition in lipid classes, monogalactosyl diacylglycerol(MGDG) digalactosyl diacylglycerol (DGDG) sulfoquinovosyl diacylglycerol (SQDG)and phosphatidyl glycerol (PG), which were isolated from 25’C-grown cells and the temperatureshifted cells, the results revealed that the double bonds were introduced into both Cl6 andCl8 fatty acids. The introduction of double bonds at A9 A12 and A6 positions yieldingpalmitoleic acid (C16:l (9)) C18:2 (9,12) and C18:3 (6,9,12) occurred in MGDG and DGDG,while the introduction of double bonds into fatty acids in SQDG and PG occurred only at A9and A12 positions. These findings suggested that the cells of S. platensis 21912 responded tolow temperature by increasing the proportion of polyunsaturated fatty acids in their membranelipids. The extent of fatty acid desaturation in S. platensis occurred firstly in plasmamembrane and then in thylakoid membrane.

มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

Address: กรุงเทพมหานคร (Bangkok)

Email: info.lib@mail.kmutt.ac.th

Name: รศ.ดร.มรกต ตันติเจริญ

Role: อาจารย์ที่ปรึกษา

Name: ผศ.บุษยา บุนนาค

Role: อาจารย์ที่ปรึกษา

Created: 2540

Issued: 2005-04-09

Modified: 2006-05-30

วิทยานิพนธ์/Thesis

ISBN: 9746240293

CallNumber: BIT200

tha

DegreeName: วิทยาศาสตรมหาบัณฑิต

Level: ปริญญาโท

Descipline: เทคโนโลยีชีวภาพ

Grantor: มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

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