การสกัดทรีฮาโลสจากยีสต์ขนมปัง

Extraction of Trehalose from Baker's Yeast

Name: นิรมล บุญยสนธิกุล

Organization : มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

keyword: Baker’s Yeast

; Extractionnrehalose

; ยีสต์ขนมปัง

; กระบวนการสกัด

; ทรีฮาโลส

Abstract: The thesis describes the study on the extraction of trehalose (C&D-glucopyranosyl1, 1 -(IL-D-glucopyranoside) from baker's yeast, S. cerevisiae. T h eresearch covered 3 parts of experiments; 1) study on the amount and stability oftrehalose in yeast cell 2) the effects of various variables such as type andconcentration of solvent, temperature, extraction time and ratio of yeast cell tosolvent, on trehalose extraction and 3) study on the suitable mode of extraction.The maximum yield of trehalose was obtained at 6 g/l00 g-DW duringculture age of 4-6 days. The yeast cells could be stored at 4 C without changingin trehalose content (approximately 5.7-6.6 g/l00 g-DW) for one month. Prolongingstorage, however, trehalose was found to decrease gradually. The content droppedto 0.27 g/l00 g-DW after 8 months.When ethanol was used as solvent, both temperature and ethanolconcentration effected the amount of trehalose extracted, The resuh and mechanismof extraction could be explained by the physico-chemical properties of solvent,solute (trehalose) and microstructure of yeast’s cell wall and membranes. Themaximum amount of trehalose extracted (6-85 g/l00 g-DW) was obtained when 50% ethanol was used as solvent and extraction temperature was controlled at 50 C.The ratio of yeast cell to ethanol at 4 g/l0 ml was suitable for extraction withoutthe problem of mixing. When 100 C pure water was used for extraction, thetrehalose extracted was only 3.6 g/l00 g-DW..The mode of extraction; Single-Stage Agitation, Multi-Stage Agitation andSoxhlet Extraction were compared by considering the criterias in the amount oftrehalose extracted, Concentration of trehalose in solvent, amount of solvent used,extraction time and efficiency. Trehalose yield 8% g/l00 g-DW with concentrationof 24 mg/10 ml solvent and 94.61 % extraction effkiency were obtained whenextracted by Single-Stage Agitation mode using 40 ml of ethanol. For Multi-StageAgitation, the trehalose yield, trehalose concentration and extraction effkiency were8.82 g/l00 g-DW, 80 mg/10 ml solvent and 93.14 % respectively. HoweverSoxhlet Extraction -gave the trehalose yield of 1.5 g/l00 g-DW and 2 1.87 %extraction efficiency were obtained. According to these results, the Multi-StageAgitation seemed to be the most appropriate mode of extraction.

มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

Address: กรุงเทพมหานคร (Bangkok)

Email: info.lib@mail.kmutt.ac.th

Name: ผศ.ดร. ดุษฏี อุตภาพ

Role: อาจารย์ที่ปรึกษา

Name: ผศ.ดร. ยุวพิน เลิศวีระวัฒน์

Role: อาจารย์ที่ปรึกษา

Created: 2539

Issued: 2005-04-09

Modified: 2006-05-30

วิทยานิพนธ์/Thesis

ISBN: 9746240048

CallNumber: BIT188

tha

DegreeName: วิทยาศาสตรมหาบัณฑิต

Level: ปริญญาโท

Descipline: เทคโนโลยีชีวภาพ

Grantor: มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

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