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Amornrat Chaikritsadakarn. Production of 5-Aminolevulinic Acid from Monosodium Glutamate Effluent by Halotolerant Photosynthetic Bacterium (Rhodobacter capsulatus SS3) and Its Application. Master's Degree(Environmental Technology). King Mongkut's University of Technology Thonburi. Library. : King Mongkut's University of Technology Thonburi, 2004.
Production of 5-Aminolevulinic Acid from Monosodium Glutamate Effluent by Halotolerant Photosynthetic Bacterium (Rhodobacter capsulatus SS3) and Its Application
Organization :
King Mongkut's University of Technology Thonburi. The Joint Graduate School of Energy and Environment. Environmental Technology
keyword:
Monosodium glutamate
;
ผงชูรส
Abstract:
Effluent from monosodium glutamate production was used as substrate for growth
and production of 5-aminolevulinic acid (ALA) from halotolerant photosynthetic
bacterium Rhodobacter capsulatus SS3 cultivated under aerobic-dark conditions at 37?C
for 4 days. The monosodium glutamate effluent (MSGE) had acidic pH (pH 3.59) and
was high in organic matter and nutrients as it contained 220,100 mgll COD, 814 mgll
reducing sugar, 3.72 % (v/v) total nitrogen, 6.18 gil Na and 11.56 % (w/w) glutamic acid.
The bacterial strain (SS3) was able to grow in MSGE, giving dry cell weight (DCW) of
3.70 gil after 36 h cultivation, but it produced a very small amount of ALA (0.20 ~M).
Both product yields were much lower than those obtained from cultivation in synthetic
medium (OSY medium), with values of 4.29 gil DCW and 40 ~M ALA. To improve
ALA production in MSOE, the effect of COD concentrations (3,426, 7,644, 15,288 mgll
and 220,100 mg/l) was studied. The optimum COD concentration was found to be 15,288
mgll, resulting in a 93-fold increase in ALA concentration to 18.6 ~M. Further
improvement of ALA production was achieved by supplementing MSOE with 40 mM
succinic acid, 5.2 mM propionic acid, 10 mM glycine, 15 mM MgCh, with the addition
of 15 mM levulinic acid (LA) at 18 h, giving a l20-fold increase in ALA concentration to
24 JlM. Application of ALA as bioherbicide was tested on giant sensitive plant (Mimosa
pigra Linn.) using 6 formulas. The optimum formula contained 5 mM ALA and 15 mM
2,2' -Dipyridyl (Dpy). In addition, application of ALA as a growth promoting factor on
Spirulina platensis strain BP was investigated. Results indicated that addition of ALA at
0.75, 1.49, 2.98, and 4.47 mM had no effect on growth (chlorophyll and cell turbidity) of
the algae.
King Mongkut's University of Technology Thonburi. Library
Address:
Bangkok
Email:
info.lib@mail.kmutt.ac.th
Name: Poonsuk Prasertsan
Role:
Advisor
Created:
2004
Modified:
2551-02-29
วิทยานิพนธ์/Thesis
application/pdf
CallNumber:
JGSEE77
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Environmental Technology
©copyrights King Mongkut's University of Technology Thonburi
RightsAccess:
ใช้เวลา
0.031904 วินาที
Amornrat Chaikritsadakarn
| Title | Contributor | Type |
|---|---|---|
| Production of 5-Aminolevulinic Acid from Monosodium Glutamate Effluent by Halotolerant Photosynthetic Bacterium (Rhodobacter capsulatus SS3) and Its Application
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี Amornrat Chaikritsadakarn | Poonsuk Prasertsan | วิทยานิพนธ์/Thesis |
Poonsuk Prasertsan