The ultrastructure of Brugia pahangi and the possible antigenic sources

ลักษณะทางจุลกายวิภาคศาสตร์ของ brugia pahangiและแหล่งแอนติเจนที่น่าจะเป็นได้

Name: Jantima Rojananin

MeSH: Antigens, Helminth

MeSH: Brugia -- ultrastructure

MeSH: Elephantiasis, Filarial

MeSH: Fluorescent Antibody Technique

MeSH: Immune Sera

MeSH: Microscopy, Electron

MeSH: Antigens

MeSH: Brugia pahangi

Abstract: The antigenie sources of adult and the third larval stage (Ls) of Brugia pahangi were detected by indirect immunofluorescent technique, using six panels of antisera, including human antisera against B. malavi and W. bancrofti, cat antisera against B.malavi and B. pahangi as primary antisera against B.malayi and B. pahangi as primary antibodies. All antisera give the same results, although four of the six were not infected by B.pahangi. This indicates the non-species specificty, and that B.pahangi, B.malavi and W.bancrofti must shore most of the common antigenic molecules. All antisera reacted well with the surface of Ls B.pahangi in the whole mount preparations, indicatint the non-stage specificity as well as non-species specificity. The most intense fluorescence was located at the epicuticle, the basal lamina lining thre body wall, the basal laminae covering the gut and the reproductive tracts, the egg shell in utero and the sperms. The hypodermis, the muscle cells, the cuticle beneath the epicuticle, the epithelial cells of the gut and the reproductive tracts showed moderate fluorescence. The least fluorescence was observed in the egg interior. Transmission electron microscopy reveals that the cuticle of the L3 and adult B.pahangi is surrounded by a discontinuous trilaminate membrane-like epicuticle which is one of the richest source of antigens because this structure is in direct contact with the host immunity, thus showing high intensity of fluorescent labelling. These cuticle is composed of fibrils arranging in several directions, which are believed to be collagen-like proteins, but comprising finer fibrils with no periodicity. These cuticular proteins may be slowly turned over and released to the environment and act as one source of immunogens. The hypodermis shows cellular components in the lateral cords, each cell bears organelles characteristics of highly synthetic activity and the infolded plasma membrane at both apical and the basal regions. Therefore, in addition to its role in synthesizing cuticular proteins, the infolded plasma membrane of the hypodermal cells may play roles in controlling and facilitating and facilitation the exchange of nutrient and waste materials through the cuticle; and excreted materials may also be another source of antigens. The somatic muscle cells comprise myofibrillar and non-myofibrillar portions, the latter sends slender processes toward the dorsal and the ventral cords which are enriched in neural elements, thus forming the interdigitating caps over the cords where nerve-muscle associations occur. The musculature, being a part of the body wall. may also permit the passages of nutritive and excretory/secretory materials in and out through it from the pseudocoel. It is also faintly stained with various antisera, thus it may contain many kinds of moderately immunogenic molecules. The epithelial cells of uterus and the vas deferens show short irregular microvilli. This implies that the gut may be poorly functioning in the ingestion and absorption of food, since these substances can as well be uptaken via the cuticle and hypodermis. The pseudocoelom function as a reservoir for both nutrients and waste, and also as a turgor pressure system. Substances may traverse from internal organs and pseudocoel across the body wall, and act as another excretory antigens. The basal laminae provide support to the organs and act as filtration barrier, as food and waste products are transported in and out. The basal laminae thus show high antigenicity that may be derived from their own compositions as well as from substances that pass through and therefore trapped in these layers.

Mahidol University. Mahidol University Library and Knowledge Center

Address: NAKHONPATHOM

Email: liwww@mahidol.ac.th

Name: Prasert Sobhon

Role: Thesis Advisors

Name: Sanjai Sangvichien

Role: Thesis Advisors

Name: Wanlop Chusattayanond

Role: Thesis Advisors

Name: Suchart Upatham

Role: Thesis Advisors

Name: Chaitip Wanichanon

Role: Thesis Advisors

Created: 1991

Modified: 2561-02-19

Issued: 2018-02-17

วิทยานิพนธ์/Thesis

application/pdf

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Anatomy

Grantor: Mahidol University

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