Studies on formation, regeneration and fruiting body formation of Pleurotus cystidiosus protoplasts

การศึกษาการสร้างโปรโตพลาสต์ การกลับคืนเป็นเส้นใยและการสร้างดอกเห็ดจากโปรโตพลาสต์ของเห็ดเป๋าฮื้อ

Name: Sudarat Boonchan

LCSH: Regeneration (Botany)

LCSH: Protoplasts

LCSH: Pleurotus

Abstract: Conditions suitable for the release of mycelial protoplasts of Pleurotus cystidfosus and for reversion of the isolated protoplasts were examined. The study culminated in the development of a method using commercially available preparation of 2.0 % Novozym(TM) 234, 1.5 % Chitinase, 1.5 % Driselase and 2.0 % Cellulase, by which about 3.8 x 10(6)/ml protoplasts could be obtained from the young growing mycelia, within 10 hours. The 0.05 M Na-maleate buffer containing 0.6 M MgSo(,4), pH 5.8 is suitable as protoplast buffer (buffer/stabilizer). Protoplasts from P. cystidiosus mycelia degenerated into normal hyphae with frequencies about 0.19 % in glucosemalt-yeast extract medium supplemented with 1.0 % sorbitol (regeneration medium), in embedding method. The reversion frequency was shown influence by nutrient composition, especially of carbon and nitrogen sources in the regeneration medium. Mycelium derived from protoplasts of P. cystidiosus consistently developed normal fruiting bodies.

Mahidol University. Mahidol University Library and Knowledge Center

Address: NAKHONPATHOM

Email: liwww@mahidol.ac.th

Name: Preecha Klingesorn

Role: Thesis Advisors

Name: Flegel, Timothy William

Role: Thesis Advisors

Created: 1991

Modified: 2018-02-16

Issued: 2018-02-16

วิทยานิพนธ์/Thesis

application/pdf

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Environmental Biology

Grantor: Mahidol University

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1	10040663-eng.pdf	301.88 KB
2	10040663-tha.pdf	166.58 KB
3	10040663.pdf	24.43 MB

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